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pAG8Ha-ETV1_DBD(332-458)

Plasmid

#188052

Purpose

Expresses His8-ETV1_DBD(332-458) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

ETV1 (ETV1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-331, 459-477

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-EWS

Plasmid

#188044

Purpose

Expresses His8-EWS with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

EWSR1 (EWSR1 Human)

Tags

His8

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD(276-399_F362A)

Plasmid

#188047

Purpose

Expresses His8-FLI1_DBD(276-399_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated F362A

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[21-881]-C-int

Plasmid

#199211

Purpose

For ubiquitous expression of split-intein Gal4-C-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13198

Plasmid

#219873

Purpose

The base plasmid of TUNEYALI forTF08

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08

Expression

Yeast

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-TO-Δ37 PPM1H-TMEM115-HA

Plasmid

#208374

Purpose

Lentiviral expression of human Δ37-PPM1H with C-terminal TMEM115-HA

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

delta37 PPM1H-TMEM115-HA (PPM1H Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta37

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12567

Plasmid

#219927

Purpose

PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter CYC

Expression

Yeast

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12566

Plasmid

#219926

Purpose

PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter DGA

Expression

Yeast

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13236

Plasmid

#219909

Purpose

The base plasmid of TUNEYALI for TF46

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46

Expression

Yeast

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13199

Plasmid

#219874

Purpose

The base plasmid of TUNEYALI forTF09

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13218

Plasmid

#219893

Purpose

The base plasmid of TUNEYALI for TF28

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13247

Plasmid

#219918

Purpose

The base plasmid of TUNEYALI for TF57

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13228

Plasmid

#219903

Purpose

The base plasmid of TUNEYALI for TF38

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NpSxph

Plasmid

#193097

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Nanorana parkeri saxiphilin

Tags

3C-eGFP-His10

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NpSxphI559Y

Plasmid

#193099

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Nanorana parkeri saxiphilin I559Y

Tags

3C-eGFP-His10

Expression

Insect

Mutation

I559Y

Promoter

polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OsSxph

Plasmid

#193100

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Oophaga sylvatica saxiphilin

Tags

3C-eGFP-His10

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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