We narrowed to 75,914 results for: Rest
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Plasmid#20486DepositorInsertFGFR1 (FGFR1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196082Purpose(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196076Purpose(Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag GCK
Plasmid#20492DepositorInsertGCK (GCK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag AKT3
Plasmid#20423DepositorInsertAKT3 (AKT3 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196080Purpose(Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Blasticidin S deaminase
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196074Purpose(Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
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EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
SHP2(WT)@pLKO-Trex-SBP
Plasmid#85457PurposeGene expressionDepositorInsertSHP2 (PTPN11 Human)
UseLentiviralTagsSBP TagExpressionMammalianMutationPromoterRSVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
SHP2(C459S)@pLKO-Trex-HA
Plasmid#85459PurposeExpresses SHP2 mutant in mammalian cellsDepositorInsertSHP2 (PTPN11 Human)
UseLentiviralTagsHAExpressionMammalianMutationC459SPromoterRSVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
SHP2(T253M/Q257L)@pLKO-Trex-SBP
Plasmid#85458PurposeSHP2 mutant gene expressionDepositorInsertSHP2 (PTPN11 Human)
UseLentiviralTagsSBP TagExpressionMammalianMutationT253M/Q257LPromoterRSVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_WDR38:1-314
Plasmid#211329PurposeMammalian expression of full-length human WDR38. C-terminal HiBiT tag.DepositorInsertWDR38:M1-T314 (WDR38 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_RBBP7:1-425
Plasmid#211113PurposeMammalian expression of full-length human RBBP7. N-terminal HiBiT tag.DepositorInsertRBBP7:M1-S425 (RBBP7 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP
Plasmid#192287PurposeExpresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4DepositorInsertUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_WDR20:1-569
Plasmid#211136PurposeMammalian expression of full-length human WDR20. N-terminal HiBiT tag.DepositorInsertWDR20:M1-P569 (WDR20 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DNAAF10:1-357
Plasmid#211138PurposeMammalian expression of full-length human DNAAF10. N-terminal HiBiT tag.DepositorInsertDNAAF10:M1-I357 (WDR92 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_PPWD1:1-646
Plasmid#211116PurposeMammalian expression of full-length human PPWD1. N-terminal HiBiT tag.DepositorInsertPPWD1:M1-K646 (PPWD1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_WDR70:1-654
Plasmid#211143PurposeMammalian expression of full-length human WDR70. N-terminal HiBiT tag.DepositorInsertWDR70:M1-I654 (WDR70 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only