We narrowed to 23,479 results for: CRISPR

1. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

   Plasmid

   #196710

   Purpose

   Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCSh3-mRFP

   Plasmid

   #170983

   Purpose

   Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   mRFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSHS248

   Plasmid

   #87354

   Purpose

   S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S/S867C/N1054C

   Promoter

   T7

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. dSp-VP64-RTA

   Plasmid

   #99675

   Purpose

   Expresses dSp Cas9 fused at C term. To VP64 and RTA activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Tags

   VP64-RTA

   Expression

   Mammalian

   Mutation

   dead Cas9

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL1P3_AtU626Ter26

   Plasmid

   #191771

   Purpose

   Cas9 guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   SpCas9 guide scaffold with AtU626 promoter/terminator
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL11197

   Plasmid

   #191784

   Purpose

   SpCas9 expression in plants

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   AtUbi10::Cas9 +13 introns-T-E9
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1820_pCR8-DR-RG6-SA-DR

   Plasmid

   #118654

   Purpose

   Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   DR-RG6-SA-DR
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas-Acr (insertion 5)

   Plasmid

   #128116

   Purpose

   Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   CascAID (insertion 5)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas-Acr (N39A)

   Plasmid

   #128117

   Purpose

   Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   CascAID (N39A)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLS234

    Plasmid

    #73682

    Purpose

    SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    GFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. NAX_CAGGS_CAS9_VPR_EYFP

    Plasmid

    #167890

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEC578

    Plasmid

    #174366

    Purpose

    dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    dCas9 fused to SYNZIP18
    

    Expression

    Bacterial

    Promoter

    J23150

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW212

    Plasmid

    #113234

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Expression

    Bacterial

    Mutation

    GGTAG->CCATC

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFETCh_SIX4_iso1

    Plasmid

    #128238

    Purpose

    Donor vector for 3' FLAG tag of human SIX4_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SIX4_iso1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEc-crRNA1

    Plasmid

    #170088

    Purpose

    encodes E. coli type I-E single spacer array

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E single spacer array
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    J23119

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. OA-1050S (UASt-dCasRx)

    Plasmid

    #132419

    Purpose

    Express dCasRx under UASt promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    CasRx
    UASt+hsp70
    

    Expression

    Insect

    Promoter

    UASt

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas-Acr (wtAcrIIA4)

    Plasmid

    #128115

    Purpose

    Expresses SpyCas9 fused to wild-type AcrIIA4

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (wtAcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBC2102-reci

    Plasmid

    #117171

    Purpose

    U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    reci_wtCas9 without NLS
    

    Use

    Lentiviral

    Promoter

    U6 Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH184

    Plasmid

    #122857

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas-Acr (D14A/G38A)

    Plasmid

    #128118

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (D14A/G38A)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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