We narrowed to 18,031 results for: Mut

1. pLentiGuide_mCherry_NT135

   Plasmid

   #183121

   Purpose

   pLentiGuide modified to express mCherry and a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLARRYv2-EGFP-T2A-Flpo

   Plasmid

   #233212

   Purpose

   Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selection

   Depositor

   Alejo Rodriguez-Fraticelli

   Article

   Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis-MBP-huErCas12a

   Plasmid

   #174685

   Purpose

   Expresses 6xHis-MBP tagged ErCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huErCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis

   Expression

   Bacterial

   Mutation

   Q926R mutation is observed as indicated from the …

   Promoter

   T7

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. prCAG-MS2

   Plasmid

   #191121

   Purpose

   Transcription of 47xCAG and 12xMS2 repeats from a tetracycline-inducible promoter

   Depositor

   Ariel Lindner

   Article

   Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

   Inserts

   rCAG
   MS2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Tet

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5B-TGFbeta receptor I K232R

   Plasmid

   #11763

   Depositor

   Jeff Wrana

   Article

   Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

   Insert

   TGFbeta receptor I (TGFBR1 Human)
   

   Use

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   K232R: kinase dead

   Promoter

   CMV

   Available Since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pRU319

   Plasmid

   #167686

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

   Plasmid

   #49772

   Purpose

   Level 2 construct

   Depositor

   Sophien Kamoun

   Article

   Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

   Insert

   sgRNA_PDS2-Cas9-sgRNA_PDS1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA6.MCV.LT339.V5.4

   Plasmid

   #28193

   Depositor

   Patrick Moore

   Article

   Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

   Insert

   MCPyV Truncated Tumor Large T antigen (399 tumor strain)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHEE901

    Plasmid

    #91707

    Purpose

    CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGE655

    Plasmid

    #153253

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR_Puro_sgAAVS1

    Plasmid

    #187818

    Purpose

    pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-neo VPS54-13Myc

    Plasmid

    #175095

    Purpose

    13Myc tagged VPS54 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Gershlick et al Hum Mol Genet. 2019 May 1;28(9):1548-156

    Insert

    VPS54 (VPS54 Human)
    

    Use

    Tags

    13Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LT2_12_GlyRNA

    Plasmid

    #177710

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRES2-EGFP Rac2

    Plasmid

    #12193

    Depositor

    Gary Johnson

    Article

    Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

    Insert

    Rac2 (RAC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET sfGFP(150TAG)

    Plasmid

    #133455

    Purpose

    expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

    Depositor

    Yu-Hsuan Tsai

    Article

    Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

    Insert

    sfGFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPN-mKate (PT7LacO-PN-mKate)

    Plasmid

    #191126

    Purpose

    Expression of protein N tagged with mKate from a T7-lac inducible promoter

    Depositor

    Ariel Lindner

    Article

    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

    Insert

    Protein N
    

    Use

    Synthetic Biology

    Tags

    mKate

    Expression

    Mutation

    Promoter

    T7-lac inducible promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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