We narrowed to 61,721 results for: Ran

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pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Plasmid

#176534

Purpose

Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associated IscB (Delaware Bay metagenome)

Tags

14xHis-MBP-bdSUMO and TwinStrep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLuc-WPRE-HGHpA

Plasmid

#87951

Purpose

Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

Insert

Firefly Luciferase

Use

AAV

Promoter

EF1a

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

Plasmid

#107313

Purpose

Encoding aCamkII-mCherry-Cre

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

Use

AAV

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP7 (102-220)

Plasmid

#42314

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Δ1-102

Promoter

CMV

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pROS10

Plasmid

#107924

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flt3-ITD-Y591F/Y919F

Plasmid

#74499

Purpose

Retroviral expression of FLT3 ITD-Y591F/Y919F

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

unknown

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMEL13

Plasmid

#107919

Purpose

kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pKGmDUET-NgBR/hCIT

Plasmid

#203173

Purpose

Expression of human cis-prenyltransferase complex for purification

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Inserts

NUS1 (NUS1 Human)
DHDDS (DHDDS Human)

Tags

Strep-Tag II-TEV and internally 6HIS tagged

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-APPSw;T719N

Plasmid

#177094

Purpose

For lentiviral transduction of APPsw; T719N

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

APPsw;T719N (APP Human)

Use

Lentiviral

Mutation

T719N, K651N and M652L (please see depositor comm…

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-JUNB

Plasmid

#192907

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of JUNB

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

JUNB (JUNB Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-YBX1

Plasmid

#192904

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of YBX1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

YBX1 (YBX1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-FUSN-VP16

Plasmid

#183933

Purpose

Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65449

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

tetO

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-mTagBFP2-rMPC1 shRNA

Plasmid

#229016

Purpose

Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Rat)

Use

Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

U6

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHDM-SARS-CoV-2 Δ18

Plasmid

#164434

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(FL)

Plasmid

#221243

Purpose

Expression of STING without transmembrane domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING without transmembrane domain (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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Gfp-zf-vasa

Plasmid

#167323

Purpose

Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

Depositor

Goro Yoshizaki

Article

Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

Insert

zf vasa 3'UTRs (ddx4 Zebrafish)

Expression

Bacterial

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAF92

Plasmid

#135929

Purpose

All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Insert

mCherry fused to TNFSF9

Use

Transposon

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pROS17

Plasmid

#107931

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

Plasmid

#171090

Purpose

CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

Depositor

Sebastian Lourido

Article

Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

Inserts

Sth1 dCas9
Chloramphenicol acetyltransferase
TagBFP

Use

CRISPR; Toxoplasma gondii expression

Tags

3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

Mutation

D9A, H599A

Promoter

TUB1 (Toxoplasma gondii), transcriptionally linke…

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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