We narrowed to 7,766 results for: loxP

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pmCellFree_KA1_ZIKV NS4A

Plasmid

#145346

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term eGFP

Depositor

Rob Parton

Publication

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

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IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pACE-K2P10.1-StrepTagII

Plasmid

#191475

Purpose

Expresses K2P10.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Publication

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P10.1 (KCNK10, TREK-2 Human)

Expression

Insect

Mutation

N144Q (uniprot P57789-1)

Promoter

polH

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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phABCA3-eGFP_CRISPR_Donor

Plasmid

#188540

Purpose

Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

Depositor

Darrell Kotton

Publication

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

EGFP

Use

CRISPR; Donor for homologous recombination

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8_MF-PH-Flag-Nter (VE5627)

Plasmid

#139773

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an N-ter Flag tag under the pH promoter.

Depositor

Arnaud Poterszman

Publication

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

N-terminal Flag tag

Tags

Flag

Promoter

PH

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-DsRed::GW,attB}

Plasmid

#182173

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::GW,attB}

Plasmid

#182172

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

eGFP

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::GW,attB}

Plasmid

#182171

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-eGFP::FRT,attB}

Plasmid

#182185

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::FRT-RC,attB}

Plasmid

#182175

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-Lyn Component 2

Plasmid

#170485

Purpose

SPELL Lyn kinase (Constitutively active) C-lobe fused to FRB. Split site is 279.

Depositor

Klaus Hahn

Publication

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FRB, Lyn(C-lobe)

Expression

Mammalian

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-INS-SM50-GFP

Plasmid

#135594

Purpose

Propogation of Insulator-SM50 Promoter-GFP DNA for microinjection to visualize Insulator-GFP promoter activity in S. purpuratus

Depositor

Koji Akasaka, Mamiko Yajima

Publication

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

INS-SM50 promoter (SM50 )

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_SARS-COV-2_NSP15

Plasmid

#169388

Purpose

Cell-free/mammalian expression vector for SARS-COV-2 virus NSP15 with N-term eGFP

Depositor

Kirill Alexandrov

Insert

SARS-COV-2 _NSP15

Use

Cell-free expression vector

Tags

His and eGFP

Promoter

T7- CMV/CAG

Available since

July 2, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4A

Plasmid

#145366

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherry

Depositor

Rob Parton

Publication

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4B

Plasmid

#145367

Purpose

Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

Depositor

Rob Parton

Publication

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAW8-aid-eDSR-Turg1:kanMX6

Plasmid

#169362

Purpose

C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe

Depositor

Tony Carr

Insert

auxin-inducible degron (aid)

Expression

Yeast

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID8Z104

Plasmid

#165056

Purpose

Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Publication

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID5VY

Plasmid

#165048

Purpose

Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Publication

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

OsTIR1 is codon-optimised for expression in S. ce…

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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1025

Plasmid

#160235

Purpose

FlightN-TetR(OFF)-VP16-Sv40-attB

Depositor

Omar Akbari

Publication

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Tetracycline repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS2A

Plasmid

#145363

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

Depositor

Rob Parton

Publication

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Tags

His and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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