We narrowed to 10,706 results for: otos

1. pLEX307-IBV-3CL-C143A

   Plasmid

   #160289

   Purpose

   Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C143A

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-RNF8-Middle-part

   Plasmid

   #64672

   Purpose

   Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   ring finger protein 8 (RNF8 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Containing only middle part of RNF8 amino acids 1…

   Promoter

   CMV

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP Kind2 S181E

   Plasmid

   #105312

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S181E

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP Kind2 S181A

   Plasmid

   #105307

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S181A

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLR5-CAG-NLS-tdiRFP670-2A-LBR

   Plasmid

   #82620

   Purpose

   Expresses NLS-tdiRFP-2A-LBR in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMXs-hGLIS1GR

   Plasmid

   #59312

   Purpose

   Forced expression of human GLIS1GR

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

   Insert

   GLIS1 (GLIS1 Human)
   

   Use

   Retroviral

   Tags

   cGR - hormone binding domain of glucocorticoid re…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1a-CCre-MBP1-WPRE

   Plasmid

   #193917

   Purpose

   Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   CCre-MBP1
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-EF1a-NCre-MBP6-WPRE

   Plasmid

   #193916

   Purpose

   Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

   Insert

   NCre-MBP6
   

   Use

   AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28-His6-Ubc9-C138A

   Plasmid

   #133911

   Purpose

   Bacterial expression of His6-Ubc9-C138A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 138 to Alanine

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSN672

    Plasmid

    #177362

    Purpose

    Expresses human KIF1A(1-393) fused with leucine zipper and His tag

    Depositor

    Shinsuke Niwa

    Article

    Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

    Insert

    KIF1A (KIF1A Human)
    

    Use

    Tags

    His tag and Leucine zipper

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ApoE2_mCh-SspB (pBS1142)

    Plasmid

    #185324

    Purpose

    For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-GOLPH3

    Plasmid

    #21688

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    last nucleotide of stop codon removed for c-termi…

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

    Plasmid

    #107731

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDestTol2-pax3a-kcnj13-IRES-EGFP

    Plasmid

    #164950

    Purpose

    Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    pax3a promoter -5.4k

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDestTol2-actinb-kcnj13-IRES-EGFP

    Plasmid

    #164941

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pECE-M2-BAIAP2 S366A

    Plasmid

    #31657

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 S366A (BAIAP2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 366 to Alanine

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pFrt-invCAG-Luc

    Plasmid

    #63577

    Purpose

    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites

    Depositor

    Ivo Huijbers

    Article

    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

    Insert

    Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
    

    Use

    Cre/Lox; Frt/flpe recombination mediated insertion

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167848

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAN19-3xFLAG-TIR1-NOSt

    Plasmid

    #108545

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR-mCherry-p2A-LIC1 G domain

    Plasmid

    #74602

    Purpose

    expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pECE beta1 R760A

    Plasmid

    #50511

    Depositor

    Kenneth Yamada

    Article

    Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

    Insert

    integrin beta1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R760A

    Promoter

    SV40

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

23. AAV-hSyn-REX-GECO0.9

    Plasmid

    #61249

    Purpose

    Expresses REX-GECO0.9 in neurons

    Depositor

    Robert Campbell

    Article

    Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

    Insert

    REX-GECO0.9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: P60R, V61W, R6…

    Promoter

    hSyn

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail

    Plasmid

    #60392

    Purpose

    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast, Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4), amino a…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

25. II-CTCG

    Plasmid

    #43337

    Depositor

    Kiran Musunuru, Chad Cowan

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Insert

    II-CTCG
    

    Use

    Bacterial cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

26. I-GGTT

    Plasmid

    #43138

    Depositor

    Kiran Musunuru, Chad Cowan

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Insert

    I-GGTT
    

    Use

    Bacterial cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pGEX6P1-human LIC1 G domain

    Plasmid

    #74598

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. IV-AAA

    Plasmid

    #43731

    Depositor

    Kiran Musunuru, Chad Cowan

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Insert

    IV-AAA
    

    Use

    Bacterial cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

29. II-ATTT

    Plasmid

    #43282

    Depositor

    Kiran Musunuru, Chad Cowan

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Insert

    II-ATTT
    

    Use

    Bacterial cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

30. FUW-M2-PPP1R12C S452A

    Plasmid

    #31679

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Protein Phosphatase 1 Regulatory Subunit 12C S452A (PPP1R12C Human)
    

    Use

    Lentiviral

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 452 to Alanine

    Promoter

    Available since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only