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pTS37.13

Plasmid

#106265

Purpose

Polycistronic expression of ekivR, luc, esyn1 using P2A

Depositor

Vera Meyer

Article

Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

Insert

ekivR-P2A-luc-P2A-esyn1

Use

Luciferase and Synthetic Biology

Promoter

Tet-On

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-G11-5FLAG-kanMX6

Plasmid

#49182

Purpose

genomic G11-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G11-5FLAG

Expression

Yeast

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-HA

Plasmid

#67171

Purpose

CRISPaint universal donor for HA fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

HA

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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JDS78

Plasmid

#32290

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Type

Empty backbone

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-SSBP2

Plasmid

#28090

Depositor

Lalitha Nagarajan

Article

Liang et al Oncogene. 2005 Apr 14. 24(16):2625-34.

Insert

SSBP2 (SSBP2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
FC858

Plasmid

#27328

Depositor

Fred Cross

Article

Cross et al Yeast. 2011 Feb . 28(2):167-79.

Insert

CDH1 (CDH1 Budding Yeast)

Expression

Yeast

Mutation

11 Phosphorylation site point mutations: T12A S16…

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pBS hBAF170

Plasmid

#17875

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF170 (SMARCC2 Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
p231 pCMVe-betaAc-STOP-luc

Plasmid

#12463

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CMVe-betaAc-STOP

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-SELP0K

Plasmid

#67007

Purpose

Expresses malE-SELP0K in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-SELP0K

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pPS811

Plasmid

#8904

Depositor

Pamela Silver

Article

Lee et al Genes Dev 1996 May 15;10(10):1233-46.

Insert

NPL3 (NPL3 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-Darpin E_01

Plasmid

#66996

Purpose

Expresses malE-Darpin E_01 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Darpin E_01

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRU1064

Plasmid

#14472

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

Gfp-UV-GusA

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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JDS71

Plasmid

#32287

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Type

Empty backbone

Use

T7

Tags

3X Flag and FOKI WT

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pJMP2849

Plasmid

#160677

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJT106b

Plasmid

#31392

Depositor

Christopher Voigt

Article

Tabor et al J Mol Biol. 2011 Jan 14. 405(2):315-24.

Insert

red light inverter

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pG6L6 CAT (P#1541)

Plasmid

#14590

Depositor

Matija Peterlin

Article

Zhang et al Genes Dev. 2003 Mar 15. 17(6):748-58.

Insert

5xGal4 and 5xLexA binding sites

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pMR150

Plasmid

#32292

Depositor

Verne Chapman, Rudolf Jaenisch

Article

Sanford et al Genes Dev. 1987 Dec;1(10):1039-46.

Insert

genomic methylation probe-minor satellite repeat

Expression

Bacterial

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pFCiK5

Plasmid

#167514

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-poly-RGD

Plasmid

#67002

Purpose

Expresses malE-poly-RGD in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly-RGD

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAB147

Plasmid

#101662

Purpose

Opto-T7RNAP*(302)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(302)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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