We narrowed to 15,991 results for: LEA

1. PcDNA-DEST53-CypB-Del3 N-term GFP

   Plasmid

   #36135

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 39-210 of cyclophilin B

   Promoter

   CMV

   Available Since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

2. Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

   Plasmid

   #15602

   Depositor

   Susan Lindquist

   Article

   Tessier et al Nature. 2007 May 31. 447(7144):556-61.

   Insert

   Sup35 NM (SUP35 Budding Yeast, Candida albicans)
   

   Tags

   7xHis and Cysteine

   Expression

   Bacterial

   Mutation

   4 G/A. Four glycines mutated to alanines. The pos…

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPS835

   Plasmid

   #8933

   Depositor

   Pamela Silver

   Article

   Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

   Insert

   GSP1 (GSP1 Budding Yeast)
   

   Tags

   myc

   Expression

   Yeast

   Mutation

   G21V, stabilizes the GTP-bound form

   Available Since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pHWBF06EC

   Plasmid

   #130827

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Use

   Gateway entry vector

   Tags

   mCherry-FLAG-HA

   Availability

   Academic Institutions and Nonprofits only

   

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5. SUN2delCC

   Plasmid

   #130836

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Tags

   mCherry-FLAG-HA

   Expression

   Plant

   Mutation

   Deletion of coiled coil domain

   Promoter

   CaMV 35S

   Availability

   Academic Institutions and Nonprofits only

   

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6. SUN2delSUNec

   Plasmid

   #130847

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Use

   Gateway entry vector

   Tags

   mCherry-FLAG-HA

   Mutation

   Deletion of SUN domain

   Availability

   Academic Institutions and Nonprofits only

   

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7. SUN2delCCec

   Plasmid

   #130848

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Use

   Gateway entry vector

   Tags

   mCherry-FLAG-HA

   Mutation

   Deletion of coiled coil domain

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 puro_shTBK1_3185

   Plasmid

   #167294

   Purpose

   Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   shRNA TBK1 (TBK1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY010 (pcDNA3.1-hAsCpf1)

   Plasmid

   #69982

   Purpose

   Expresses humanized AsCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hAsCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SB100X in pCAG globin pA

    Plasmid

    #127909

    Purpose

    Sleeping Beauty Transposase expression vector driven by the CAG promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    Sleeping Beauty Transposase
    

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. eGFP-hCOL1A1

    Plasmid

    #140110

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag, K20…

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ADAM17-HA

    Plasmid

    #65105

    Purpose

    Expression of HA tagged ADAM17 (TACE) in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM17 (ADAM17 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFYF1548 EMX1

    Plasmid

    #47508

    Purpose

    human gRNA expression vector targeting EMX1

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EMX1 (EMX1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_Pro-IL-1α

    Plasmid

    #214341

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1A (IL1A Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBad-EBFP2

    Plasmid

    #14891

    Purpose

    Bacterial expression of EBFP2 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Has Service

    Cloning Grade DNA

    Insert

    EBFP2
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMVTNT-T7-KPNA7

    Plasmid

    #26683

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 7 (KPNA7 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV sgRNA-Hic1 GFP

    Plasmid

    #106953

    Purpose

    Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    anti-Hic1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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