We narrowed to 14,258 results for: cas9

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pMEL11

Plasmid

#107917

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWR63(YUM1)

Plasmid

#203336

Purpose

episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence

Use

CRISPR

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR63

Plasmid

#203331

Purpose

episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)

Use

CRISPR

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_KLF4

Plasmid

#140104

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_NFE2

Plasmid

#140105

Purpose

CRISPR-Cas9 Knock down

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_TREML2

Plasmid

#140106

Purpose

CRISPR-Cas9 Knock down

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_LINC02432

Plasmid

#140107

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_MIR3945HG

Plasmid

#140108

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_Intergenic

Plasmid

#140100

Purpose

CRISPR-Cas9 library validation (negative control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_FURIN

Plasmid

#140103

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA sCRNA 2.0 (GB2461)

Plasmid

#160593

Purpose

Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA sCRNA 2.0

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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LC-E18

Bacterial Strain

#115924

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Bacterial Resistance

None

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only
FR-E01

Bacterial Strain

#118727

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Bacterial Resistance

None

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-ack

Plasmid

#62654

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Expression

Bacterial

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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EC67842

Plasmid

#209516

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC67841

Plasmid

#209512

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC67843

Plasmid

#209513

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ183

Plasmid

#98425

Purpose

Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ184

Plasmid

#98426

Purpose

Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBFv-U6.2

Plasmid

#138400

Purpose

gRNA expression vector for Drosophila

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Type

Empty backbone

Expression

Insect

Promoter

U6-2

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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