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pCMV-mGold2s-Keratin-N-17

Plasmid

#231767

Purpose

Visualization of Keratin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Keratin-N-17 (KRT18 Human)

Tags

mGold2s

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Keratin-N-17

Plasmid

#231775

Purpose

Visualization of Keratin in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Keratin-N-17 (KRT18 Human)

Tags

mGold2s

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pME AmCyan (JDW 1151)

Plasmid

#224485

Purpose

Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

AmCyan

Use

Gateway Cloning

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME V5 mScarlet-I (JDW 1323)

Plasmid

#224487

Purpose

Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway Cloning

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME iCre-I (JDW 1203)

Plasmid

#224520

Purpose

A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

iCre-I

Use

Cre/Lox

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD1 N-terminal sgRNA

Plasmid

#207101

Purpose

pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATGGCAGGACTATGGCAGCC

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM C-terminal sgRNA

Plasmid

#207097

Purpose

pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTCTAAAGGCTGAATGAAA

Expression

Mammalian

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.b - pRSET

Plasmid

#193948

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Publication

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Tags

His-Tag

Expression

Bacterial

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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GreenT-EC.c - pRSET

Plasmid

#193949

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Publication

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.c

Tags

His-Tag

Expression

Bacterial

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_110

Plasmid

#180554

Purpose

Entry vector containing 7xhis tag in E1.3b format

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

7xhis tag in E1.3b format

Expression

Bacterial

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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miniCMV-mCerulean-tDeg

Plasmid

#185401

Purpose

mCerulean-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Publication

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

mCerulean-tDeg

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSP566

Plasmid

#139500

Purpose

Gene tagging vector to add mCherry Tag and membrane anchoring motif

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

mCherry-CaaX

Expression

Yeast

Mutation

WT

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_loxP

Plasmid

#62430

Purpose

MXS_chaining vector with loxP

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

loxP site

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pWZLFlagMenin(S394A, T397A, S399A)

Plasmid

#32086

Depositor

Matthew Meyerson

Publication

Francis et al PLoS One. 2011 . 6(1):e16119.

Insert

Menin

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

S394A, T397A, S399A

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-Soma-jGCaMP8f (AAV Retrograde)

Viral prep

#169258-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from AAV-hSyn-Soma-jGCaMP8f (#169258). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8f plasmid DNA. Synapsin-driven neuronal expression of soma-targeted calcium sensor jGCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Marianne Fyhn

Publication

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-DIO-dLight3.6

Plasmid

#257860

Purpose

Cre-dependent expression of the green genetically encoded dopamine sensor dLight3.6 in mammalian cells under CAG promoter.

Depositor

Lin Tian

Publication

Roshgadol et al Res Sq [Preprint]. 2025 Aug 20:rs.3.rs-7

Insert

dLight3.6

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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