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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 4761 - 4780 of 6962 results

  1. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid
    #137828
    Purpose
    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-CD28TMD-NTEVp(H75S)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Histidine 75 to Serine in NTEVp fragment
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137828-plasmid-map-sequence-id-311924

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  4. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid
    #135355
    Purpose
    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEMax-2XNLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135355-plasmid-map-sequence-id-267704

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  5. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid
    #136451
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Tags
    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136451-plasmid-map-sequence-id-271690

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  6. pcDNA3-HCMVgH (TB40/E)

    Plasmid
    #136446
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136446-plasmid-map-sequence-id-271694

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  7. pcDNA3-sACE2-WT(732)

    Plasmid
    #154098
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 (untagged)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154098-plasmid-map-sequence-id-304580

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  9. mCerulean-hCOL1A1

    Plasmid
    #140112
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140112-plasmid-map-sequence-id-296778

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  10. pcDNA3-sACE2v2-Fc(IgA1)

    Plasmid
    #145157
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgA1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145157-plasmid-map-sequence-id-292593

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  11. pcDNA3-sACE2v3-sfGFP

    Plasmid
    #145174
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    145174-plasmid-map-sequence-id-292600

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  12. HA-RNF126 C231A

    Plasmid
    #138648
    Purpose
    Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)
    Depositor
    Jane McGlade
    Publication
    Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNF126 (RNF126 Mouse)
    Tags
    HA-HA
    Expression
    Mammalian
    Mutation
    C231A
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    138648-plasmid-map-sequence-id-273239

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  17. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid
    #126425
    Purpose
    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
    Expression
    Mammalian
    Mutation
    alanine-6 of TdT has been changed to a STOP codon
    Promoter
    pCMV
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126425-plasmid-map-sequence-id-252600

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  19. pTBL210

    Plasmid
    #126451
    Purpose
    Expresses Cas9-XTEN-TdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126451-plasmid-map-sequence-id-245970

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  20. GFP-TIS11B CPM2

    Plasmid
    #121163
    Purpose
    Mammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)
    Depositor
    Christine Mayr
    Publication
    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZFP36L1 (TIS11B) charge pattern mutant 2
    Expression
    Mammalian
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121163-plasmid-map-sequence-id-234889

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Showing: 4761 - 4780 of 6962 results