We narrowed to 7,251 results for: tet
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Plasmid#236126PurposePlasmid encoding ADAR2dd attached to PUF-9R under control of CMV promoter with two TetR binding sitesDepositorInsertADAR2dd-PUF-9R (ADARB1 Synthetic, Human)
UseTagsExpressionMammalianMutationE488Q, T501A in ADAR2ddPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW499 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(N2093W, T2098L)
Plasmid#236146PurposePlasmid encoding the NZF transcriptional activation domain attached by a deimmunized linker to FRB with N2093W and T098L mutations, under control of CMV promoter with two TetR binding sitesDepositorInsertNZF-deImmunLink-FRB
UseTagsExpressionMammalianMutationN2093W, T2098L in FRBPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Cyr61-rDMR
Plasmid#235692PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Cyr61 with luciferase assayDepositorInsertCyr61 (Ccn1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Cyr61-rDMRdeltaTEAD
Plasmid#235693PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Cyr61 lacking Tead-binding motif with luciferase assayDepositorInsertCyr61 (Ccn1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctgf-rDMR
Plasmid#235696PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Ctgf with luciferase assayDepositorInsertCtgf (Ccn2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctgf-rDMRdeltaTEAD
Plasmid#235697PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Ctgf lacking Tead-binding motif with luciferase assayDepositorInsertCtgf (Ccn2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Myc-rDMR
Plasmid#235694PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Myc with luciferase assayDepositorInsertMyc (Myc Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA FRT to BioID-cDIC1-3xFlag
Plasmid#232417PurposeExpresses human dynein subunit DIC1 in mammalian cells.DepositorInsertDynein Intermediate Chain 1 (DYNC1I1 Human)
UseTagsBioID and Flag-3xExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y
Plasmid#226349PurposeExpression of Titin V15P substrate with GS tailDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10
Plasmid#226351PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5
Plasmid#226352PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1
Plasmid#226353PurposeExpression of titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mVprBP pc2953
Plasmid#201698PurposeExpresses of GFP-tagged VprBP in mammalian cellsDepositorInsertVprBP (Dcaf1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmcherry-mVprBP pc2954
Plasmid#201699PurposeExpresses of mcherry-tagged mVprBP in mammalian cellsDepositorInsertVprBP (Dcaf1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
hARL15_32-197
Plasmid#209799PurposeBacterial expression of human ARL15 GTPase domainDepositorInsertARL15 (ARL15 Human)
UseTags6xHisExpressionBacterialMutationaa 32-197 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENeCFPCNAL2 pc922
Plasmid#167563PurposeFor construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(363-393)
Plasmid#200314Purposebacterial expression of P53 CTDDepositorInsertp53(363-393) (TP53 Human)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLA2ScIfmTcIfm
Plasmid#193792PurposeCassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertTwo copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLLacO-1 and PLTetO-1Available sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid with onboard mCherry cassette
Plasmid#169735PurposeEqualizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARB366
Plasmid#124049PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only