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Plasmid#66258PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCX3C1 (CX3CR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPR68-Tango
Plasmid#66371PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR68 (GPR68 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
HCA1-Tango
Plasmid#66395PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHCA1 (HCAR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
LTB4R-Tango
Plasmid#66423PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertLTB4R (LTB4R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-CD28 CAR hinge (I114 – P152)
Plasmid#224929PurposeExpression GST fusion CD28 CAR hinge (I114-P152) in Escherichia coli.DepositorInsertCD28 CAR hinge (I114 – P152) (TMIGD2 Human)
UseTagsglutathione-S-transferaseExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR68-DuET
Plasmid#213290PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR68 (GPR68 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R(T308A)
Plasmid#208092PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A mutation with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R(T308A) (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationT308APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R(S310A)
Plasmid#208093PurposeMammalian expression of the human LTB4 receptor LTB4R with the S310A mutation with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R(S310A) (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationS310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R(T308A,S310A)
Plasmid#208094PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R(T308A,S310A) (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationT308A, S310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR68
Plasmid#106031PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR68; Opto-GPR68; C9)DepositorInsertOpto-GPR68 (GPR68 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only