Addgene

We narrowed to 998 results for: Hexa

Showing: 461 - 480 of 998 results

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P18-pZE-SUMO-BuTTA

Plasmid

#204632

Purpose

ColE1 ori, KanR, TetR, Tet promoter with a codon optimized buTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

Depositor

Aditya Kunjapur

Article

Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

Insert

His-SUMO-TEV-BuTTA

Use

Synthetic Biology

Tags

His Tag; SUMO Tag; TEV Protease Site

Expression

Bacterial

Mutation

Promoter

PLtetO-1 promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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P24-pZE-SUMO-KaTTA

Plasmid

#204633

Purpose

ColE1 ori, KanR, TetR, Tet promoter with a codon optimized kaTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

Depositor

Aditya Kunjapur

Article

Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

Insert

His-SUMO-TEV-KaTTA

Use

Synthetic Biology

Tags

His Tag; SUMO Tag; TEV Protease Site

Expression

Bacterial

Mutation

Promoter

PLtetO-1 promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet1-LanCL2-H264A-C-Ter-His

Plasmid

#154187

Purpose

To express LanCL2-H264A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

His264->Ala264

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1-H219A

Plasmid

#154190

Purpose

To express LanCL1-H219A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

His219->Ala219

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1-C322A

Plasmid

#154191

Purpose

To express LanCL1-C322A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys322->Ala322

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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12XCSL-d1EGFP

Plasmid

#47684

Purpose

Notch reporter

Depositor

Urban Lendahl

Article

Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Insert

TP-1 promoter

Use

Notch reporter construct

Tags

d1EGFP

Expression

Mutation

hexamerized 50-bp EBNA2 response element of the T…

Promoter

NA

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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12XCSL-DsRedExpressDL

Plasmid

#47683

Purpose

Notch reporter

Depositor

Urban Lendahl

Article

Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Insert

TP-1 promoter

Use

Notch reporter construct

Tags

dsRedExpressDR

Expression

Mutation

hexamerized 50-bp EBNA2 response element of the T…

Promoter

NA

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-SaNAS

Plasmid

#110803

Purpose

Expresses SaNAS a nicotianamine synthase from Staphylcoccus aureus

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

SAV2469 (SAV2469 )

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-PaNAS

Plasmid

#110801

Purpose

Expresses PaNAS nicotianamine synthase from pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4836

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-SaODH

Plasmid

#110804

Purpose

Expresses SaODH and opine dehydrogenase from Staphylococcus aureus

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

SAV2468 (SAV2468 )

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-AlpJ

Plasmid

#139598

Purpose

expresses AlpJ in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

alpJ

Use

Tags

N terminal hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinO1

Plasmid

#139597

Purpose

expresses KinO1 in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

kinO1

Use

Tags

N terminal hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1::ADAT3-ADAT2

Plasmid

#15917

Depositor

Nina Papavasiliou

Article

Rubio et al Proc Natl Acad Sci U S A. 2007 May 8. 10

Insert

ADAT3 & ADAT2

Use

Tags

FLAG and HexaHistidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR TERRA

Plasmid

#111383

Purpose

TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA

Use

Gateway entry cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-PaODH

Plasmid

#110802

Purpose

Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4835

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF H407A H409A H448A

Plasmid

#139566

Purpose

expressed SznF H407A H409A H448A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H407A H409A H448A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X_MBP-CLmN-H6

Plasmid

#234487

Purpose

Maltose binding protein N-terminally fused to His-tagged CLmN split intein

Depositor

Henning Mootz

Article

Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

Insert

N-terminal Fragment of the CLm Split Intein

Use

Tags

Hexahistidine Tag and Maltose Binding Protein (MB…

Expression

Bacterial

Mutation

T69K, F75H, M118N within the N-terminal fragment …

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.PHF1

Plasmid

#125167

Purpose

Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF1 (PHF1 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163

Plasmid

#127192

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 (FUS Human)

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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