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pEGFP-C1-FLAG-GFP-Ubl4A

Plasmid

#86921

Purpose

To express Ubl4A tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET23a-HsUBB

Plasmid

#69562

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (UBB Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pAXY0006

Plasmid

#205575

Purpose

Glucos(1-231)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Inserts

Glucosyltransferase and NpuDnaE(N)_Arabidopsis-codon-OP
CYP76AD1-P2A-DODA

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUF-AsCpf1-pre-gRNA

Plasmid

#137849

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1 and pre-gRNA cassette

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-GFP

Plasmid

#106948

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1 yeBFP2-T2A-mCherry

Plasmid

#219851

Purpose

equimolar expression of yeBFP2 and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

yeBFP2-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yomTagBFP2-T2A-mCherry

Plasmid

#205493

Purpose

Expression of yomTagBFP2-T2A-mCherry in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

yomTagBFP2-T2A-mCherry

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pThuS

Plasmid

#171674

Purpose

ThuS expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

Glycosyltransferase ThuS

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pUF-LbCpf1-pre-gRNA

Plasmid

#137848

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

LbCpf1-NLS-FLAG and gRNA cassette

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YE1

Plasmid

#154145

Purpose

Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE1 (dCas12a-BE-YE1)

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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REM2

Plasmid

#55682

Purpose

expression of mouse REM2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM2 (Rem2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pDRF1 mScarlet1.4-T2A-mTurquoise2

Plasmid

#219849

Purpose

equimolar expression of mScarlet1.4 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

mScarlet1.4-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 mStayGold-T2A-mCherry

Plasmid

#219834

Purpose

equimolar expression of mStaygold and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

mStayGold-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1 Cyterm-ytd8oxStayGold

Plasmid

#219853

Purpose

expression of Cyterm-ytd8oxStayGold in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

Cyterm-ytd8oxStayGold

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RIT1

Plasmid

#55662

Purpose

expression of mouse RIT1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RIT1 (Rit1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 F201A

Plasmid

#26728

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

F201A: GEF domain mutant

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
RASD1

Plasmid

#55671

Purpose

expression of mouse RASD1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASD1 (Rasd1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pDRF1 ymStayGold-T2A-mCherry

Plasmid

#219843

Purpose

equimolar expression of ymStayGold and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymStayGold-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 L196A

Plasmid

#26727

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

L196A: GEF domain mutant.

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pBT138b-pro1

Plasmid

#138513

Purpose

P3h

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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