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pU6-sgRNA_MET_uORF2

Plasmid

#216538

Purpose

gRNA and template for MET 5'UTR uORF2 mutation

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

pegRNA MET 5'UTR uORF2 mutation

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-uORF1-MET

Plasmid

#214104

Purpose

MET 5'UTR upstream of mGFP (uORF1 mutated)

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

MET 5'UTR

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

uORF1 mutation

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBz84

Plasmid

#226318

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis- (wtMR1-6)- strep

Tags

6xHis and Strep

Expression

Bacterial

Mutation

N-terminal domain deleted, C-terminal domain dele…

Promoter

T7

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_MYC9

Plasmid

#223897

Purpose

4G-cloning donor for C-terminal MYC9 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

Myc9

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_PK6

Plasmid

#223898

Purpose

4G-cloning donor for C-terminal PK6 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

PK6

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG

Plasmid

#223895

Purpose

4G-cloning donor for C-terminal FLAG tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_HALO

Plasmid

#223899

Purpose

4G-cloning donor for C-terminal HALO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

HALO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6

Plasmid

#223871

Purpose

4G-cloning donor for N-terminal His6 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6

Use

Golden-gate donor

Tags

6xHis

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-3C

Plasmid

#223873

Purpose

4G-cloning donor for N-terminal His6-3C tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_HALO

Plasmid

#223885

Purpose

4G-cloning donor for N-terminal HALO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

HALO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP

Plasmid

#223880

Purpose

4G-cloning donor for N-terminal GFP tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

GFP

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_TS-3C

Plasmid

#223883

Purpose

4G-cloning donor for N-terminal TwinStrep-3C tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

TwinStrep-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP-3C

Plasmid

#223882

Purpose

4G-cloning donor for N-terminal GFP-3C tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

GFP-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_His8

Plasmid

#223886

Purpose

4G-cloning donor for C-terminal His8 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His8

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_TS

Plasmid

#223891

Purpose

4G-cloning donor for C-terminal TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-TS

Plasmid

#223892

Purpose

4G-cloning donor for C-terminal 3C-TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3C-TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk7

Plasmid

#208991

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk7 (kcnk7 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pML33

Plasmid

#206996

Purpose

Contains OsTiR1 ORF and LoxP and Lox2272 sites.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

OsTiR1

Expression

Mammalian

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pML58

Plasmid

#206997

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and hygromycin b resistance gene.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

HygR

Use

Unspecified

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pML60

Plasmid

#206998

Purpose

Contains mAID-tag, mouse Ptbp1-specific homology arms and puromycin resistance gene.

Depositor

Eugene Makeyev

Article

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

PuroR

Use

Unspecified

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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