We narrowed to 22,490 results for: SCR

1. pHAGE EF1α dCas9-VP64

   Plasmid

   #50918

   Purpose

   Constitutive dCas9-VP64 lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1alpha

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNDL-60

   Plasmid

   #161720

   Purpose

   In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

   Depositor

   Adam Cohen

   Article

   McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

   Insert

   cytosolic acvr2b-CIB1N (acvr2b Zebrafish)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSFV-Helper1

   Plasmid

   #92073

   Purpose

   This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

   Depositor

   Henrik Garoff

   Article

   Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

   Insert

   cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
   

   Use

   Helper plasmid, for run-off transcription and rna…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

   Plasmid

   #61762

   Purpose

   Dual Color betaGlobin reporter to study splicing kinetics in vivo

   Depositor

   Daniel Larson

   Article

   Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

   Insert

   b-Globin
   

   Use

   Tags

   24 X MS2 hairpin cassette in 3'UTR of b-Glob…

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible Tet-ON promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-PV-NES-GFP

   Plasmid

   #17301

   Depositor

   Anton Bennett

   Article

   Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

   Insert

   PV-NES-GFP (Pvalb Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Fra-2

   Plasmid

   #187907

   Purpose

   Expresses Fra-2 in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   Fra2 (Fosl2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCGN-SRF

   Plasmid

   #11977

   Purpose

   For mammalian expression of human SRF with an HA tag

   Depositor

   Ron Prywes

   Article

   Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

   Insert

   SRF (SRF Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBMNb-cALFA

   Plasmid

   #159991

   Purpose

   For the protein expression with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   CMV promoter, WPRE

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDA083

    Plasmid

    #131389

    Purpose

    eGFP expression

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PPRE-H2B-eGFP

    Plasmid

    #84393

    Purpose

    Fluorescent reporter for PPARgamma activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

    Insert

    PPARG response element (PPARG Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PPRE

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1-puro U6 sgRNA BfuAI stuffer

    Plasmid

    #50920

    Purpose

    U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRPN265NB-LaG16-MNase

    Plasmid

    #170978

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

    Insert

    Glutathione-S-Transferase/LaG16/MNase
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmIL-6 FL

    Plasmid

    #61286

    Purpose

    drives luciferase from Wt mouse IL-6 promoter (full length)

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IL-6 full length promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NylCK_pET28a

    Plasmid

    #215422

    Purpose

    Expression construct for NylCK gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCI pHluorin Syt7

    Plasmid

    #184508

    Purpose

    Mammalian expression of Synaptotagmin 7 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 7 (Syt7 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHMGCS-Luc (aka: pES8)

    Plasmid

    #14947

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    HMG CoA synthase promoter
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGAL4-DBD-MCS

    Plasmid

    #145245

    Purpose

    Backbone vector expressing GAL4 DNA-binding domain followed by MCS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. T7-RelA(KR)

    Plasmid

    #23251

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    NFkB RelA (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    K218/221R, K310R

    Promoter

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pPB-PGK-destination

    Plasmid

    #60436

    Purpose

    Destination vector with PGK - Hygromycin selection marker

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lenti-sgRNA-BSD

    Plasmid

    #175583

    Purpose

    Expresses sgRNA for CasRx in mammalian cell

    Depositor

    Qi Xie

    Article

    Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

    Plasmid

    #112925

    Purpose

    Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6 promoter, gRNA-SAM expression cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP

    Plasmid

    #119903

    Purpose

    Bxb1 integration plasmid for cuO array with Puro selection cassette

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    cuOx144 array
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SpCas9

    Plasmid

    #188489

    Purpose

    Expresses FLAG tagged SpCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p-LCN2

    Plasmid

    #28225

    Depositor

    Sebastien Jauliac

    Article

    Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

    Insert

    LCN2 promoter (LCN2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    LCN2

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pINDUCER20-Flag-hSPDEF WT

    Plasmid

    #100873

    Purpose

    Lentiviral expression of human SPDEF

    Depositor

    Noah Shroyer

    Article

    Lo et al Gastroenterology. 2017 Jul;153(1):205-21

    Insert

    SPDEF (SPDEF Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE2

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LentiGuide Cherry

    Plasmid

    #170510

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Pavel Tolar

    Article

    Newman et al biorXiv 2021.03.02.433398

    Inserts

    S. pyogenes sgRNA cassette
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CROP_C9_Puro_ERBB2

    Plasmid

    #183287

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    ERBB2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. OPN(-1206)-luc

    Plasmid

    #31106

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    osteopontin promoter (SPP1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    promoter region -1206 to +87

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. hTLR3-pcDNA3

    Plasmid

    #32712

    Depositor

    Saumen Sarkar

    Article

    Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

    Insert

    TLR3 (TLR3 Human)
    

    Use

    Tags

    FLAG and Preprototrypsin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBW103ParsR-Amp30C

    Plasmid

    #78638

    Purpose

    pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    30hrpR-30hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Flag-NRF1-HA

    Plasmid

    #34708

    Depositor

    Raymond Deshaies

    Article

    Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

    Insert

    NRF1 (NFE2L1 Human)
    

    Use

    Retroviral

    Tags

    3XFlag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVS2

    Plasmid

    #33144

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Inserts

    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GFP is mutated such that amino acids 64-73 (FSYGV…

    Promoter

    bidirectional GAL1-GAL10 promoter

    Available since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113343

    Purpose

    Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    p300core-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMRX-IZ-HaloTag7-CHMP4A

    Plasmid

    #184895

    Purpose

    Stable expression of HaloTag7-CHMP4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUPD2+p35SoTMV

    Plasmid

    #170876

    Purpose

    Phytobrick - p35SoTMV

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    p35SoTMV
    

    Use

    Tags

    Expression

    Plant

    Mutation

    214C>T and 229C>T to remove BbsI restrictio…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. IVT-R2Tg_opt

    Plasmid

    #223247

    Purpose

    In vitro transcription of the optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge