We narrowed to 25,968 results for: Vit;

1. pAc-C Med12His

   Plasmid

   #49240

   Purpose

   expresses human Med12 with His tag in insect cells

   Depositor

   Dylan Taatjes

   Article

   Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

   Insert

   Med12 (MED12 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB-rDA2h

   Plasmid

   #208701

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA2h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Circular 200,100 (mPCSK9)

   Plasmid

   #170122

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. P-rTetO-EGFP-T2A-RPL22-3xHA

   Plasmid

   #170326

   Purpose

   Tetracycline-inducible expression of HA and EGFP tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

   Plasmid

   #170317

   Purpose

   Expresses HA and EGFP tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-FLEX-ArchT-tdTomato

   Plasmid

   #28305

   Purpose

   Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Has Service

   AAV5

   Insert

   ArchT-tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Neo_CAG-Cas9

   Plasmid

   #86698

   Purpose

   Cas9 expression vector for targeting to the AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCag

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPR77-DuET

   Plasmid

   #213291

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR77 (C5AR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

   Plasmid

   #170327

   Purpose

   Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG87 FAST

    Plasmid

    #130719

    Purpose

    Expresses His-tagged FAST (also called YFAST) in bacteria

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    FAST
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLJM1-Flag-Rheb-4Rs

    Plasmid

    #165022

    Purpose

    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb-4Rs (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    change lysine residues on position 109, 135, 151,…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. STK11 gRNA (BRDN0001146880)

    Plasmid

    #75912

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GIPR-DuET

    Plasmid

    #213252

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GIPR (GIPR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH-CMV-RPS4X-3xHA plasmid

    Plasmid

    #192246

    Purpose

    overexpression vector of RPS4X-3XHA

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    RPS4X CDS without stop coden (RPS4X Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

    Plasmid

    #170320

    Purpose

    Expresses HA and mCherry tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP457 pGK-dCas9-Suntag-BFP

    Plasmid

    #100957

    Purpose

    BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA and BFP

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-GRAB-rDA2mut

    Plasmid

    #208705

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) control sensor GRAB_rDA2mut in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) control sensor GRAB_rDA2mut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-TRE-DIO-Casp3-TEV

    Plasmid

    #183766

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFPN1-N-gamma-tubulin (334-449)

    Plasmid

    #87859

    Purpose

    Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment from aa 334 to 449

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. His-GIV-CTs

    Plasmid

    #69864

    Purpose

    Express His tagged GIV-CTs (1660–1870).

    Depositor

    Pradipta Ghosh

    Article

    Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86.

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C terminal amino acids S1660-S1870 of human GIV.

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-FLEX-ArchT-GFP

    Plasmid

    #28307

    Purpose

    Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

    Plasmid

    #170321

    Purpose

    Expresses HA and mCherry tagged RPL22, neomycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TRIM28 gRNA (BRDN0001162487)

    Plasmid

    #76139

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLVX-hSyn-Galpha-s ONE-GO

    Plasmid

    #214371

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TBK1 gRNA (BRDN0001148607)

    Plasmid

    #76363

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FRB-GFP-Gab1(Y628F/Y660F)

    Plasmid

    #188658

    Purpose

    FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    GRB associated binding protein 1 (Gab1 Mouse)
    

    Use

    Tags

    FRB and GFP

    Expression

    Mammalian

    Mutation

    Y628F and Y660F

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPBbsr2-Gnaq

    Plasmid

    #170298

    Purpose

    Encoding mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A recombinant mouse Gnaq (Gnaq Mouse)
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Asilent mutation was introduced into the sgRNA-ta…

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ADORA2B-DuET

    Plasmid

    #213175

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADORA2B (ADORA2B Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TRIM28 gRNA (BRDN0001145369)

    Plasmid

    #76141

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pScalps_Puro_mCebpa

    Plasmid

    #79551

    Purpose

    Expression of mouse Cebpa

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Cebp alpha (Cebpa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFastBacI-KDAC6

    Plasmid

    #224346

    Purpose

    Expresses human KDAC6 (HDAC6) in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC6 (HDAC6 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pLenti HsATP13A3 D498N

    Plasmid

    #195850

    Purpose

    Expresses D498N mutant Homo sapiens ATP13A3

    Depositor

    Veerle Baekelandt

    Article

    Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

    Insert

    ATPase 13A3 D498N (ATP13A3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. BDKBR2-DuET

    Plasmid

    #213190

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    BDKBR2 (BDKRB2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. STK11 gRNA (BRDN0001146194)

    Plasmid

    #75913

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV_FKBP-V5-TWIST1

    Plasmid

    #215022

    Purpose

    AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Use

    AAV

    Tags

    FKBP-V5

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TRIM28 gRNA (BRDN0001146162)

    Plasmid

    #76142

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. STK11 gRNA (BRDN0001147319)

    Plasmid

    #75914

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ADORA1-DuET

    Plasmid

    #213173

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADORA1 (ADORA1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-EphB3-Fc

    Plasmid

    #200985

    Purpose

    Mammalian expression plasmid for soluble EphB3 fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB3 (EPHB3 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Soluble ectodomain of EphB3

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge