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Showing: 7231 - 7260 of 8782 results

  1. SAGA040

    Plasmid
    #240789
    Purpose
    Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 His medium : T7- His tags medium- gfp -RK2 tetARK2 - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240789-plasmid-map-sequence-id-484214

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  2. SAGA039

    Plasmid
    #240788
    Purpose
    Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240788-plasmid-map-sequence-id-484210

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  3. pRECMYC-Tsp1

    Plasmid
    #240712
    Purpose
    pLAM12 vector backbone containing Tsp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Tsp11 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240712-plasmid-map-sequence-id-482753

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  4. pRECMYC-Kva1

    Plasmid
    #240709
    Purpose
    pLAM12 vector backbone containing Kva1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Kva1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240709-plasmid-map-sequence-id-482751

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  5. pRECMYC-Hcl1

    Plasmid
    #240714
    Purpose
    pLAM12 vector backbone containing Hcl1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Hcl1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240714-plasmid-map-sequence-id-482749

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  6. pRECMYC-Eas1

    Plasmid
    #240710
    Purpose
    pLAM12 vector backbone containing Eas1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eas1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240710-plasmid-map-sequence-id-482711

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  11. pRECMYC-Csp1

    Plasmid
    #240711
    Purpose
    pLAM12 vector backbone containing Csp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Csp1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240711-plasmid-map-sequence-id-482471

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  12. pRECMYC-Abe1

    Plasmid
    #240708
    Purpose
    pLAM12 vector backbone containing Abe1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Abe1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240708-plasmid-map-sequence-id-482469

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  13. pRECMYC-Ban1

    Plasmid
    #240707
    Purpose
    pLAM12 vector backbone containing Ban1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Ban1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240707-plasmid-map-sequence-id-482428

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  18. pREC1010-Tsp1

    Plasmid
    #240687
    Purpose
    RSF1010 origin of replication plasmid containing Tsp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Tsp1 RT, Tsp1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240687-plasmid-map-sequence-id-482372

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  19. pREC1010-Hcl1

    Plasmid
    #240689
    Purpose
    RSF1010 origin of replication plasmid containing Hcl recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Hcl1 RT, Hcl1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240689-plasmid-map-sequence-id-482371

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  20. pREC1010-Ebu1

    Plasmid
    #240688
    Purpose
    RSF1010 origin of replication plasmid containing Ebu1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240688-plasmid-map-sequence-id-482363

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  21. pREC1010-Ban1

    Plasmid
    #240682
    Purpose
    RSF1010 origin of replication plasmid containing Ban1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Ban1 RT, Ban1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240682-plasmid-map-sequence-id-482353

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  22. pREC1010-Eas1

    Plasmid
    #240685
    Purpose
    RSF1010 origin of replication plasmid containing Eas1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eas1 RT, Eas1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240685-plasmid-map-sequence-id-482350

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  23. pREC1010-Csp1

    Plasmid
    #240686
    Purpose
    RSF1010 origin of replication plasmid containing Csp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Csp1 RT, Csp1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240686-plasmid-map-sequence-id-482344

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  24. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

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  27. pQdCas12a.sggfp(A)

    Plasmid
    #236038
    Purpose
    The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236038-plasmid-map-sequence-id-469933

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  28. pQdCas12a.sggfp(B)

    Plasmid
    #236040
    Purpose
    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236040-plasmid-map-sequence-id-469924

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Showing: 7231 - 7260 of 8782 results