We narrowed to 69,572 results for: CaS;

1. pX330-H11r1-2-DD-Cas9

   Plasmid

   #85978

   Purpose

   Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBLO 9.6_bCas9-CP1010

   Plasmid

   #121498

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBLO 9.15_bCas9-CP1249

   Plasmid

   #121500

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLO 9.16_bCas9-CP1282

   Plasmid

   #121501

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p15A

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbdCas9G(RBSmut)_arr2

   Plasmid

   #149578

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaSpeR-act5cB-LexAQFw

   Plasmid

   #61311

   Purpose

   Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   LexA_DBD::QF2w_AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Changed EK on C-terminus of QF2 to KKKK

   Promoter

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbdCas9K(-10TC)_arr2

   Plasmid

   #149584

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbdCas9K(RBSmut)_arr2

   Plasmid

   #149577

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBSKΔB-CBh-Cas9n-pA

   Plasmid

   #82617

   Purpose

   Expresses Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   SpCas9n
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC1-pCR8-dCas9VP48

    Plasmid

    #48214

    Purpose

    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP48 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP48

    Expression

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-LmoCascade-IL1RNcrRNA

    Plasmid

    #126493

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo IL1RN cr03
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-LmoCascade-HBE1crRNA

    Plasmid

    #126494

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo HBE1 cr06
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-FLAG-eSpCas9

    Plasmid

    #126769

    Purpose

    Expression of increased fidelity eSpCas9 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCaSpeR3/ncd-gfp #10

    Plasmid

    #66082

    Purpose

    stable germline transformation of Drosophila to produce Ncd-GFP expression

    Depositor

    Sharyn Endow

    Article

    Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.

    Insert

    Ncd kinesin-14 motor protein (ncd Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    D699L

    Promoter

    ncd promoter (3.1kb upstream region of ncd start …

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pRK5-HA CASTOR2 ACT2

    Plasmid

    #84494

    Purpose

    overexpression of CASTOR2 ACT2 domain (aa 171-end)

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 ACT2 (GATSL2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    171-last amino acid

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-HA CASTOR1 ACT2

    Plasmid

    #84492

    Purpose

    overexpression of CASTOR1 ACT2 domain (aa 169-end)

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 ACT2 (GATSL3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    169-last amino acid

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG189 CASC2 3'UTR

    Plasmid

    #12017

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N21 3'UTR (CASC2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RCAS(A) cWnt14 (CT#693)

    Plasmid

    #13948

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    Wnt-14 (WNT9A Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. TDK promoter dCas9

    Plasmid

    #113657

    Purpose

    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    TDK gene promoter and dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TDK gene promoter

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+) Laccase2 Sense

    Plasmid

    #69892

    Purpose

    Expresses the Drosophila laccase2 exon 2 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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