We narrowed to 118,634 results for: MPI;

1. pCMV-NFIB2ERt2

   Plasmid

   #112703

   Purpose

   Expression of NFIB2-ERT2 fusion protein

   Depositor

   Richard Gronostajski

   Insert

   Nuclear Factor I B2 (Nfib Mouse)
   

   Tags

   ERT2 and HA

   Expression

   Mammalian

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. C98m

   Plasmid

   #121002

   Purpose

   MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Gp2 toxin
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FgH1tUTG_Bfl-1.1

   Plasmid

   #85536

   Purpose

   Inducible expression of guide RNA (Bfl-1.1) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Insert

   Bfl1.1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   H1t

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAST Ag83b

   Plasmid

   #53443

   Purpose

   UAS-Anopheles gambiae Orco in pUAST Vector

   Depositor

   Leslie Vosshall

   Insert

   pUAST Ag83b (NEWENTRY Anopheles gambiae)
   

   Expression

   Insect

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

5. CAG-EGFP-MosIR

   Plasmid

   #120516

   Purpose

   dsRNA expression

   Depositor

   Petr Svoboda

   Insert

   Mos (Mos Mouse)
   

   Use

   RNAi

   Mutation

   L170P in second MosIR repeat (see comments below)

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. C51m

   Plasmid

   #120975

   Purpose

   MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   sfYFP (has BbsI site)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. C33m

   Plasmid

   #120968

   Purpose

   MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   AiiA degradase (Bennett, no ssra tag)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREP3xN_ccdB

   Plasmid

   #79021

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. KCNK10C-c011

   Plasmid

   #53639

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KCNK10C
   

   Use

   Baculovirus expression

   Tags

   TEV-His10-Flag

   Promoter

   polyhedrin

   Available Since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A APGST -XE

    Plasmid

    #113839

    Purpose

    Histone H2B-superfolder GFP and tdTomato linked by T2A (APGST linker -XE)

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Expression

    Mammalian

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK678

    Plasmid

    #72376

    Purpose

    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene

    Depositor

    T. J. Kappock

    Insert

    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    

    Use

    Gene synthesis

    Promoter

    n/a

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-DJ1-E18Q

    Plasmid

    #29404

    Depositor

    Mark Cookson

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Glu18Gln

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pDEST40-DJ1-E18D

    Plasmid

    #29403

    Depositor

    Mark Cookson

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    E18D

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

14. L4781

    Plasmid

    #1679

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, rf2204, ATG-MitLS, gfpN, IVSR, gfpGLA, IVSS…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458_TGIF2_1

    Plasmid

    #72374

    Purpose

    Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    TGIF2 (TGIF2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_TGIF2_2

    Plasmid

    #72375

    Purpose

    Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    TGIF2 (TGIF2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-loxP-3xpA-H2BsfGFP-tdTomato - P2A -APGST XE

    Plasmid

    #113854

    Purpose

    Histone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-P2A-tdTomato
    

    Expression

    Mammalian

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. piRES-puro-HE4

    Plasmid

    #25727

    Depositor

    Ronny Drapkin

    Insert

    WAP four-disulfide core domain 2 (WFDC2 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pColdII-SIRPT1-Nelumbo nucifera

    Plasmid

    #75358

    Purpose

    Nt His-tag Nelumbo nucifera Sacsin 1-1313

    Depositor

    Kalle Gehring

    Insert

    Sacsin Nelumbo nucifera 1-1313
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD95_03

    Plasmid

    #1479

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, SV40NLS, lacZN, IVSB, IVSD, IVSE, IVSF, IVS…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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