We narrowed to 9,286 results for: Pol;

1. pET21a - EtfA

   Plasmid

   #89616

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Electron transport flavoprotein alpha subunit
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-PC-1-CTS

   Plasmid

   #86067

   Purpose

   A bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Polycystin-1 (PKD1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains the region from 4297-4303

   Promoter

   tac

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21a - TDR

   Plasmid

   #89627

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   thiol dependent reductase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21a - NFDD

   Plasmid

   #89624

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   NAD/FAD dependent dehydrogenase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBP-J23108

   Plasmid

   #72964

   Purpose

   Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23108 promoter
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBP-J23114

   Plasmid

   #72965

   Purpose

   Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23114 promoter
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBP-T7_RBS

   Plasmid

   #72978

   Purpose

   T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7+RBS
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTU2-A-lacZ

   Plasmid

   #72950

   Purpose

   Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - A site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTU2-B-lacZ

   Plasmid

   #72951

   Purpose

   Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - B site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTU2-C-lacZ

    Plasmid

    #72952

    Purpose

    Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - C site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTU2-D-lacZ

    Plasmid

    #72953

    Purpose

    Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - D site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTU2-B-RFP

    Plasmid

    #72955

    Purpose

    Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - B site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTU2-C-RFP

    Plasmid

    #72956

    Purpose

    Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - C site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTU2-D-RFP

    Plasmid

    #72957

    Purpose

    Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - D site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTU2-b-RFP

    Plasmid

    #72959

    Purpose

    Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - b site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

16. CAG-mCherry-RORbshRNA2

    Plasmid

    #73984

    Purpose

    RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-EGFP-RORbshRNA1

    Plasmid

    #73985

    Purpose

    RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CAG-EGFP-RORbshRNA2

    Plasmid

    #73986

    Purpose

    RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse RORb gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CAG-mCherry-Otx2shRNA2

    Plasmid

    #73980

    Purpose

    Otx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 ZFYVE9

    Plasmid

    #67134

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ZFYVE9 (ZFYVE9 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    F1359I

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only