We narrowed to 5,703 results for: Feng Zhang

1. pX330-NEAT1pr_v2

   Plasmid

   #97082

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1pr_v2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-NEAT1m_v1

   Plasmid

   #97083

   Purpose

   Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1m_v1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX335-NEAT1_IS_v1

   Plasmid

   #97085

   Purpose

   Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1_IS_v1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX335-NEAT1_IS_v2

   Plasmid

   #97086

   Purpose

   Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1_IS_v2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-LbCpf1-U6_FlipArray

   Plasmid

   #109349

   Purpose

   Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

   Insert

   U6-FlipArray
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-GFP-SFPQ

   Plasmid

   #97084

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA GFP-SFPQ
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p202_LTJ_sgRNACD45.2_R1

   Plasmid

   #82672

   Purpose

   sgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting mouse CD45.2 region 1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459V2.0+K848A

   Plasmid

   #108296

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K848A mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. px459-Rheb sgRNA

   Plasmid

   #133768

   Purpose

   Expresses Cas9 and human Rheb sgRNA

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   Rheb sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PX330-sgRNA006_CTCF

    Plasmid

    #232928

    Purpose

    Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9 (Ctcf S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX459-sg-CMV152

    Plasmid

    #179916

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX459-sg-CMV51

    Plasmid

    #179915

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX459-sg-CMV162

    Plasmid

    #179917

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459-sg-mGreenLantern332

    Plasmid

    #179914

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p236_LTJ_sgRNAFoxp3sf/J

    Plasmid

    #82676

    Purpose

    sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting Foxp3 sf/J
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cdk12_intron3_sg2_pX330

    Plasmid

    #127174

    Purpose

    sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cdk12 Intron 3 sgRNA
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cdk12_intron4_sg1_pX458

    Plasmid

    #127175

    Purpose

    sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cdk12 Intron 4 sgRNA
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pANY1

    Plasmid

    #110949

    Purpose

    Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.

    Depositor

    Yingfeng An

    Article

    Liu et al Biochem Biophys Res Commun. 2018 Mar 18;

    Insert

    ccd B cassette
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-U6-sgRNA-hSyn-mCherry

    Plasmid

    #87916

    Purpose

    sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

    Depositor

    Alex Hewitt

    Article

    Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GRAB_DA1m (AAV9)

    Viral Prep

    #113049-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_DA1m (#113049). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_DA1m plasmid DNA. Synapsin-driven expression of GRAB-DA1m dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

    Promoter

    Syn

    Tags

    None

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only