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pNK885

Plasmid

#219733

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1824x

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn1824x

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn1824x

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK878

Plasmid

#219734

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1904x

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn1904x

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn1904x

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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C1C2CMUNC2C

Plasmid

#218674

Purpose

Insect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2C

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

MUNC13 (Unc13a Rat)

Tags

GST

Expression

Insect

Mutation

Amino acids1407-1453 deleted

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-AC17-5c-frGFP

Plasmid

#217525

Purpose

AC17-5c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Article

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

AC17-5c riboswitch-regulated folding reporter GFP

Use

In vitro transcription-translation

Promoter

T7

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS160

Plasmid

#215682

Purpose

Guide only plasmid targeting chrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIBA2-IntHA453-SpyTag

Plasmid

#198039

Purpose

Expression plasmid for producing SpyTagged intimin

Depositor

Jack Leo

Article

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

double HA tag inserted at position 453; lacks nat…

Promoter

tet

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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mTB3-FLAG tag

Plasmid

#210204

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

FLAG-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mTB3-AviD tag

Plasmid

#210203

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

AviD-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Bmp7

Plasmid

#201909

Purpose

in vitro transcription of RNA encoding FLAG-tagged Bmp7a

Depositor

Mary Mullins

Article

Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

Insert

bmp7 (bmp7a Zebrafish)

Tags

FLAG tag

Promoter

SP6

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.26_4xUAS_DSCP_GFP

Plasmid

#111931

Purpose

Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

4xUAS GFP

Expression

Insect

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pE5771

Plasmid

#170778

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

Kan resistance (kanR )

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pE5773

Plasmid

#170780

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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hp-66%GC-40bp

Plasmid

#169200

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

66%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-75%GC-20bp

Plasmid

#169201

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

75%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-75%GC-40bp

Plasmid

#169202

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

75%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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