Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,099 results for: CAD;

Showing: 4881 - 4900 of 10099 results

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CRISPRi sgNF1-1

Plasmid

#253096

Purpose

CRISPRi single-guide against NF1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

NF1 (NF1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgSOS1-1

Plasmid

#253092

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-ΔHsFBXO28-Myc-MBP-HA

Plasmid

#228946

Purpose

Expression ΔHsFBXO28-Myc (lacking F-box domain) and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

ΔHsFBXO28 (FBXO28 Human)

Tags

myc

Expression

Bacterial

Mutation

deletion of the F-box domain

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAH_DEST_nHalo-Sec61β-3XFLAG

Plasmid

#250996

Purpose

Expression of Sec61β with N-terminal Halo and C-terminal 3XFLAG tags

Depositor

Anthony Pedley

Article

Sha et al Proc Natl Acad Sci U S A. 2026 Feb 3;123

Insert

SEC61β (SEC61B Human)

Tags

3XFLAG and Halo Tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pMX Bmal1pomoter mVenus-linkerA-BMAL1

Plasmid

#249337

Purpose

Fluorescence reporter for intracellular distribution of BMAL1 protein

Depositor

Takeaki Ozawa

Article

Tamaru et al Commun Biol. 2025 Dec 17. doi: 10.1038/s

Insert

BMAL1 (Bmal1 Mouse)

Use

Retroviral

Tags

Venus

Promoter

Bmal1 promoter

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pMX Bmal1pomoter mVenus-linkerB-BMAL1

Plasmid

#249338

Purpose

Fluorescence reporter for intracellular distribution of BMAL1 protein

Depositor

Takeaki Ozawa

Article

Tamaru et al Commun Biol. 2025 Dec 17. doi: 10.1038/s

Insert

BMAL1 (Bmal1 Mouse)

Use

Retroviral

Tags

Venus

Promoter

Bmal1 promoter

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Article

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9 K1151TAG, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHCF/V5

Plasmid

#247002

Purpose

Expresses HCF-1 with carboxy terminal V5 tag under the control of the CMV promoter

Depositor

Thomas Kristie

Article

Vogel et al Proc Natl Acad Sci U S A. 2006 May 2;103

Insert

HCF-1 (HCFC1 Human)

Tags

V5 tag

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hCCDC32-HA

Plasmid

#243756

Purpose

Express human CCDC32 in mammalian cells with HA tag at C terminus.

Depositor

Jingshi Shen

Article

Wan et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Coiled-coil domain-containing protein 32 (CCDC32 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(K55R)-Avi

Plasmid

#245186

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

K55R

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(T52S)-Avi

Plasmid

#245185

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

T52S

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(L43F)-Avi

Plasmid

#245184

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

L43F

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(T42A)-Avi

Plasmid

#245183

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

T42A

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(WT)-Avi

Plasmid

#245182

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

wild-type

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-C-SH2(R138Q)-Avi

Plasmid

#245189

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 C-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

R138Q

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(E76K)-Avi

Plasmid

#245187

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

E76K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-C-SH2(WT)-Avi

Plasmid

#245188

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 C-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

wild-type

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-C-SH2(E139D)-Avi

Plasmid

#245190

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 C-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

E139D

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/T119M

Plasmid

#242712

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, T119M

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/V122I

Plasmid

#238122

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, V122I

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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