We narrowed to 9,802 results for: otos
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Plasmid#44985DepositorInsertFUS (Full length) (FUS Human)
UseTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b-DiCas7-11-His
Plasmid#234482PurposePlasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)DepositorInsertDiCas7-11
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
M23: pHR-SFFVp-FUSN::mCherry::SspB
Plasmid#122148PurposeN terminal domain of FUS (1-214) tagged with SspB and mCherry.DepositorInsertFUS (FUS Human)
UseLentiviralTagsmCherry-sspBExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-9K0Q
Plasmid#221739PurposeStable expression of GFP-9K0Q in mammalian cells to monitor membrane chargeDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-170PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorInsertGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
UseTagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB
Plasmid#122668PurposeC terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.DepositorInsertHNRNPA1 (HNRNPA1 Human)
UseLentiviralTagsmCherry-SspBExpressionMammalianMutationContains only amino acids 186-230PromoterSFFVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-Pericam
Plasmid#87381PurposeRetroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.DepositorInsertMito-Pericam [ratiometric]
UseRetroviralTagsExpressionMammalianMutationPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-M2rtTA
Plasmid#60843PurposeAAVS1 donor vector for genomic targetingDepositorInsertsM2rtTA
Neo
UseTargeting donorTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4-AARE-luc2P-Hygro
Plasmid#101787PurposeLuferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)DepositorInsert3xAARE
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag
Plasmid#216695PurposeSynchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)DepositorInsertsUseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.iAChSnFR
Plasmid#137955PurposeExpresses iAChSnFR under CAG promoterDepositorArticleHas ServiceAAV1 and AAV9InsertiAChSnFR
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HDM_SARS2_Spike_del21_D614G
Plasmid#158762PurposeMammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614DepositorInsertSARS2-Spike_del21_D614G (S Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsExpressionMammalianMutationdeleted amino acids 1257-1278, changed aspartic a…PromoterCMVAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral
Plasmid#207507PurposeThis plasmid can be used to generate retrovirus.DepositorInserttNGFR, HA-GD2-28z_CAR (NGFR Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMVtrunc sfCherry2ΔC4-Lifeact
Plasmid#231557PurposeMammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopyDepositorInsertLifeact (ABP140 Budding Yeast)
UseTagssfCherry2ExpressionMammalianMutationPromoterCMVtruncAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mGold2s
Plasmid#231765PurposeExpression of mGold2s in bacterial cellsDepositorInsertmGold2s
UseTagsExpressionBacterialMutationmGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterSynthetic promoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccB
Plasmid#158113Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccA
Plasmid#158114Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EWSR1::WT1(-KTS)
Plasmid#220565PurposeLentiviral expression of human EWSR1::WT1(-KTS)DepositorUseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EWSR1::WT1(+KTS)
Plasmid#220566PurposeLentiviral expression of human EWSR1::WT1(+KTS)DepositorUseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAi14-luc-MC
Plasmid#87113Purposeminicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITIDepositorInsertLuciferase-SV40pA
UseCRISPR and LuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H188
Plasmid#170349PurposemT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RKIP_RFP
Plasmid#199215PurposeRKIP (PEBP1) expression plasmid with a RFP reporter on the backbone.DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMV and PGKAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP3Kv-WPRE
Plasmid#132332Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vectorDepositorInsertASAP3Kv
UseAAVTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-FLAG-AKAP-Lbc
Plasmid#67571PurposeExpresses GFP and FLAG-tagged AKAP-Lbc in mammalian cellsDepositorInsertA-kinase anchoring protein Lbc (AKAP13 Human)
UseTagsFLAG and GFPExpressionMammalianMutationR2604G in AKAPPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIG-829_HA-GD2-28z_CAR_JUN_Retroviral
Plasmid#207506PurposeThis plasmid can be used to generate retrovirus.DepositorInsertJUN, HA-GD2-28z_CAR (JUN Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
M31: pHR-SFFVp- TDP-43C::mCherry::SspB
Plasmid#122669PurposeC terminal domain of TDP43 (218-414) tagged with SspB and mCherry.DepositorInsertTDP43 (TARDBP Human)
UseLentiviralTagsmCherry-SspBExpressionMammalianMutationContains only amino acids 218-414PromoterSFFVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3-WPRE
Plasmid#132318Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS-WT
Plasmid#44978DepositorInsertFUS (Full length) (FUS Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
KIF5A-GFP-SspB
Plasmid#214401PurposeExpresses fusion of Kinesin heavy chain 5A (1-572) with GFP and SspB (micro)DepositorInsertKIF5A-GFP-SspB (Kif5a Synthetic, Rat)
UseTagsKIF5A-GFP-SspBExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only