We narrowed to 7,256 results for: tet

1. RalA (G23V)(mature peptide)-pcw107

   Plasmid

   #64642

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G23V

   Promoter

   PGK

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pinducer 20 DN-KASHΔPPPL

   Plasmid

   #129280

   Purpose

   Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

   Depositor

   Daniel Conway

   Article

   Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Lentiviral

   Tags

   mcherry

   Expression

   Mutation

   the last 4 amino acids (PPPL) of the KASH domain …

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro

   Plasmid

   #176850

   Purpose

   lentiviral vector for doxycycline-inducible YAP1 overexpression

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   hYAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full length YAP1

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.puro_shGLS

   Plasmid

   #110335

   Purpose

   shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Article

   Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

   Insert

   GLS glutaminase (GLS Human, Synthetic)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA PolIII)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. JAK2 (V617F)-pcw107-V5

   Plasmid

   #64610

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   JAK2 (JAK2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   V617F

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

   Plasmid

   #193769

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1ΔR198A/R210A (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPC1-hVAP-A KD/MD

   Plasmid

   #104449

   Purpose

   Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed K94 to D and M96 to D

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

   Plasmid

   #65458

   Purpose

   Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   tTA2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFPC1-hVAP-B KD/MD

   Plasmid

   #104450

   Purpose

   Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Use

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   changed K87 to D and M89 to D

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57-Cx26-IRES-GCaMP6s

    Plasmid

    #188236

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJB2 (GJB2 Human)
    

    Use

    Lentiviral

    Tags

    GCaMP6s

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Notch3 intracellular domain-pcw107-V5

    Plasmid

    #64623

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pSPL3-hRPH3A_c.444Gwt

    Plasmid

    #190476

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW-GemininΔDbox-HA-Puro

    Plasmid

    #193770

    Purpose

    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    GemininΔDbox-HA (GMNN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Dbox removed (R23A, L26A). Codons switched at siR…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TGFbetaR1 (T204D)-pcw107-V5

    Plasmid

    #64629

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    TGFBR1 (transcript variant 1) (TGFBR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    T204D

    Promoter

    PGK

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pHOT-P2Y2R

    Plasmid

    #159108

    Purpose

    entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

    Depositor

    Fabio Mammano

    Article

    Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

    Insert

    P2Y2R
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW-ND-Cdt1-mCherry-Puro

    Plasmid

    #193766

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    ND-Cdt1-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSPL3-hRPH3A_c.1853Gmut

    Plasmid

    #190479

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.1853A>G

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Caspase-8 (C360A)-pcw107-V5

    Plasmid

    #64632

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CASP8 (transcript variant B) (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    C360A

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. GSK3beta (K85A)-pcw107-V5

    Plasmid

    #64614

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    K85A

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pDam-Myc-HP1

    Plasmid

    #59221

    Purpose

    expresses Dam-myc-HP1

    Depositor

    Bas van Steensel

    Article

    van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

    Insert

    HP1 (Su(var)205 Fly)
    

    Use

    Damid

    Tags

    Dam and myc

    Expression

    Insect

    Mutation

    Promoter

    Drosophila heat shock promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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