We narrowed to 5,092 results for: codon optimized

1. pC178: pAAV.U6.SapI.CMV.Cas13bt3

   Plasmid

   #204215

   Purpose

   Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Insert

   Cas13bt3 and U6.SapI sgRNA cloning site
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Codon optimisation by GenScript tool

   Promoter

   CMV/U6

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197062

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   Encapsulin
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197063

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   Encapsulin
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-AN-DDK_3KA + S580A POLI

   Plasmid

   #131241

   Purpose

   Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert Pol iota se…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190034

   Purpose

   vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A134V,A140V,A141V,A143V

   Promoter

   CBh, SV40, hU6

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190033

   Purpose

   vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13X
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y672A,Y676A

   Promoter

   CBh, SV40, hU6

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191797

   Purpose

   vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13d
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   CBh, SV40, hU6

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223 SARS-CoV-2 NSP12

   Plasmid

   #141265

   Purpose

   Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

   Plasmid

   #223123

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9=D10A

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

    Plasmid

    #223124

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid

    #223136

    Purpose

    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized PEmax-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

    Expression

    Mammalian

    Mutation

    M-MLV mutations from PE2 (Anzalone et al. Nature …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS SARS-CoV-2 Delta Spike

    Plasmid

    #185593

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 Delta Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 Delta Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19R, Δ156-157,…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDONR223 SARS-CoV-2 NSP5

    Plasmid

    #141259

    Purpose

    Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCAGGS SARS-CoV-2 Alpha Spike

    Plasmid

    #185691

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 Alpha Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: Δ69-70, Δ145, N…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. LeGO-iG-CSF2RB(A455D)

    Plasmid

    #125751

    Purpose

    Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

    Depositor

    Dirk Heckl

    Article

    Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

    Insert

    CSF2RB(A455D)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    A455D, codon optimized

    Promoter

    SFFV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-LETMD1

    Plasmid

    #132294

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    LETMD1 (LETMD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pDONR223 SARS-CoV-2 S 24nt-del

    Plasmid

    #153952

    Purpose

    Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    June 23, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDONR223 SARS-CoV-2 S 24nt-del_nostop

    Plasmid

    #153953

    Purpose

    Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    June 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop

    Plasmid

    #153956

    Purpose

    Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    June 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDONR223 SARS-CoV-2 ORF7B C-trunc

    Plasmid

    #153957

    Purpose

    Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    June 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge