We narrowed to 10,022 results for: transfer

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pdest N-FLAG METTL1-WT puro

Plasmid

#223055

Purpose

METTL1-WT gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-WT (METTL1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-CM Neo

Plasmid

#223061

Purpose

METTL1-CM gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-CM (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

160-163 (LFPD>AFPA)

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:FRS2_CE_pISceI

Plasmid

#223040

Purpose

Express FRS2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

FRS2 (FRS2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-PH-BTKx1

Plasmid

#183651

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Human, Frog)

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Mammalian

Mutation

Amino acids 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP-INPP5E(D556A)

Plasmid

#183678

Purpose

Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

INPP5E (INPP5E Human)

Tags

mRFP-FKBP12(1-108)

Expression

Mammalian

Mutation

C641A, D556A

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2028

Plasmid

#97001

Purpose

Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-CD16-TLR4-HA

Plasmid

#246906

Purpose

Expresses CD16-TLR4 chimera in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

CD16-TLR4 (TLR4, FCGR3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

N terminus of CD16, C terminus of TLR4

Promoter

CMV

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pdest myr-AKT2 puro

Plasmid

#223053

Purpose

myr-AKT2 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

myr-AKT2 (Akt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27A Neo

Plasmid

#223059

Purpose

METTL1-S27A gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27A (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27A

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27D Neo

Plasmid

#223060

Purpose

METTL1-S27D gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27D (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27D

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest myr-AKT2 Neo

Plasmid

#223063

Purpose

myr-AKT2 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

myr-AKT2 (Akt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CCT2_CE_pISceI

Plasmid

#223041

Purpose

Express CCT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

CCT2 (CCT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CNOT_CE_pISceI

Plasmid

#223046

Purpose

Express CNOT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

CNOT2 (CNOT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-S27A_CE_pISceI

Plasmid

#223048

Purpose

Express METTL1-S27A gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27A (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

S27A

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-S27D_CE_pISceI

Plasmid

#223049

Purpose

Express METTL1-S27D gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27D (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

S27D

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-CM_CE_pISceI

Plasmid

#223050

Purpose

Express METTL1-CM gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-CM (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

160-163 (LFPD>AFPA)

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CYP27B1_CE_pISceI

Plasmid

#223028

Purpose

Express CYP27B1 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

CYP27B1 (CYP27B1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:AVIL_CE_pISceI

Plasmid

#223032

Purpose

Express AVIL gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

AVIL (AVIL Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:HMGA2_CE_pISceI

Plasmid

#223034

Purpose

Express HMGA2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

HMGA2 (HMGA2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_hRNMT_RAM

Plasmid

#213528

Purpose

Expresses the human RNMT-RAM complex

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Inserts

human RNMT (RNMT Human)
RAM

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14

Plasmid

#213518

Purpose

Expresses NSP14 of SARS-CoV-2

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

N-terminal His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

Plasmid

#213522

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed V290 to A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

Plasmid

#213523

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed N306 to K

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

Plasmid

#213524

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to S

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-EZH1-Y727F

Plasmid

#203582

Purpose

Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

Insert

EZH1 (EZH1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

changed Tyrosine 727 to Phenylalanine for partial…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-EZH1-dPSET

Plasmid

#203586

Purpose

Expresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

Insert

EZH1 (EZH1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

deletion of aa 744-747 and partial resistance to …

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-EZH1-D745N

Plasmid

#203585

Purpose

Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

Insert

EZH1 (EZH1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

changed Aspartic Acid 745 to Asparagine for parti…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il13 mutDRACH

Plasmid

#207130

Purpose

Luciferase vector containing 3'UTR for mouse Il13 with mutated DRACH sites

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 13 (Il13) 3'UTR (Il13 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

TGAGGAGAGACCATCCCTGGGCATCTCAGCTGTGGACTCATTTTCCTTT…

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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