We narrowed to 273,596 results for: addgene

1. pET28b(+)-HP0377 C92A

   Plasmid

   #39335

   Depositor

   SeWon Suh

   Insert

   oxidoreductase
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   changed Cysteine 92 to Alanine

   Promoter

   T7

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

2. HA-CREST (C89)

   Plasmid

   #22961

   Depositor

   Anirvan Ghosh

   Insert

   CREST (Ss18l1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

3. XE90 pCS2P+ XCtBP

   Plasmid

   #16704

   Depositor

   Randall Moon

   Insert

   XCtBP (ctbp2 Frog)
   

   Use

   Xenopus expression

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pRGH-12

   Plasmid

   #12222

   Depositor

   Sung-Hou Kim

   Insert

   pRas189 Gly12 truncated
   

   Expression

   Bacterial

   Mutation

   the wild type was truncated with HindIII to 171aa…

   Available Since

   Sept. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pRLH-12

   Plasmid

   #12232

   Depositor

   Sung-Hou Kim

   Insert

   pRas189 Leu12 truncated
   

   Expression

   Bacterial

   Mutation

   truncated with HindIII to 171aa (from 189aa); Gly…

   Available Since

   Sept. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pRS415-SSM1

   Plasmid

   #11003

   Depositor

   Francisco Malagon

   Insert

   SSM1 (SDT1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Available Since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. YEplac181-SSM1

   Plasmid

   #11002

   Depositor

   Francisco Malagon

   Insert

   SSM1 (SDT1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Available Since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-OMP25

   Plasmid

   #141150

   Purpose

   Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   OMP25 (Synj2bp Rat)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   OMP25 c terminus from addgene plasmid #69598

   Promoter

   CMV

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenSyn2-EGFP-­OMP25C

   Plasmid

   #71427

   Purpose

   Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

   Depositor

   Henri Huttunen

   Article

   Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

   Insert

   OMP25 (172-­‐206) (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 172-206 only

   Promoter

   synapsin

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pLVET-IRES-GFP

    Plasmid

    #107139

    Purpose

    A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

    Depositor

    Aki Manninen

    Article

    Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCasTet-λ

    Plasmid

    #128318

    Purpose

    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

    Depositor

    Christopher Johnston

    Article

    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

    Insert

    TetR and pTetR/TetO
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    Constitutive expression of cas9 and anhydro-tetra…

    Promoter

    tet promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVtight-UPRT-T2A-RFP-IRES-CD

    Plasmid

    #126677

    Purpose

    Doxycycline inducible expression of UPRT and CD along with reporter RFP

    Depositor

    Joan Massague

    Article

    Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

    Insert

    UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
    

    Promoter

    tight TRE

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    TaU3

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1-FHC-POLQ

    Plasmid

    #64875

    Purpose

    lentiviral expression of human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGM

    Plasmid

    #71250

    Purpose

    luciferase reporter with human GM-CSF promoter -627 to + 28

    Depositor

    Peter Cockerill

    Article

    Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

    Insert

    GM-CSF promoter -627 to +28 fragment (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. TMPRSS3_pCSdest

    Plasmid

    #53839

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    TMPRSS3 (TMPRSS3 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

18. MAC_N_CDK8

    Plasmid

    #111685

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CDK8 (CDK8 Human)
    

    Expression

    Mammalian

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LTN1_pCSdest

    Plasmid

    #53855

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    LTN1 (LTN1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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