We narrowed to 5,143 results for: codon optimized

1. pCAGGS SARS-CoV-2 Alpha Spike

   Plasmid

   #185691

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 Alpha Spike (S )
   

   Expression

   Mammalian

   Mutation

   Contains the following mutations: Δ69-70, Δ145, N…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available Since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. LeGO-iG-CSF2RB(A455D)

   Plasmid

   #125751

   Purpose

   Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

   Depositor

   Dirk Heckl

   Article

   Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

   Insert

   CSF2RB(A455D)
   

   Use

   Lentiviral

   Mutation

   A455D, codon optimized

   Promoter

   SFFV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-LETMD1

   Plasmid

   #132294

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   LETMD1 (LETMD1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDONR223 SARS-CoV-2 S 24nt-del

   Plasmid

   #153952

   Purpose

   Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S (S SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 23, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDONR223 SARS-CoV-2 S 24nt-del_nostop

   Plasmid

   #153953

   Purpose

   Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S (S SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop

   Plasmid

   #153956

   Purpose

   Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDONR223 SARS-CoV-2 ORF7B C-trunc

   Plasmid

   #153957

   Purpose

   Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

   Plasmid

   #223110

   Purpose

   pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpG-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

   Plasmid

   #228251

   Purpose

   pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
   

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

    Plasmid

    #127343

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Flag- HA- Tandem Epitopes

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    None

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-Neo-TetO-Cdk13_Untagged

    Plasmid

    #127344

    Purpose

    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    None

    Expression

    Mammalian

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    TetO Promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR8/GW_TOPO_Cdk13_Untagged

    Plasmid

    #127342

    Purpose

    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    None

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUt-mNF-BACH1

    Plasmid

    #199219

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV D2ir promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a(+) NRP1 b1 (273-427)

    Plasmid

    #162734

    Purpose

    Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

    Depositor

    Brett Collins

    Article

    Daly et al Science. 2020 Oct 20. pii: science.abd30

    Insert

    Human NRP1 b1 domain residues 273-427
    

    Expression

    Bacterial

    Mutation

    Codon optimised

    Available Since

    April 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET28a(+) NRP1 b1b2 (273-586)

    Plasmid

    #162733

    Purpose

    Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

    Depositor

    Brett Collins

    Article

    Daly et al Science. 2020 Oct 20. pii: science.abd30

    Insert

    Human NRP1 b1b2 domains (residues 273-586)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Codon optimised

    Available Since

    March 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pX020

    Plasmid

    #167146

    Purpose

    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal caffeoylpiruvate hydrolase, nnCPH
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX131

    Plasmid

    #167149

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    4'-phosphopantetheinyl transferase, npgA
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX018

    Plasmid

    #167144

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal luciferase, nnLuz
    

    Use

    Luciferase and Synthetic Biology

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX019

    Plasmid

    #167145

    Purpose

    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal hispidin-3-hydroxylase, nnH3H
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX022

    Plasmid

    #167148

    Purpose

    MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    C-part of fungal hispidin synthase, nnHispS
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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