We narrowed to 15,165 results for: pET

1. LgBiT-ST

   Plasmid

   #207633

   Purpose

   A plasmid encoding LgBiT fused to SpyTag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   LgBiT-SpyTag
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-Tev-UbE2D3

   Plasmid

   #186805

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE2D3 (UBE2D3 Human)
   

   Tags

   His6-Tev

   Expression

   Bacterial

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK_kanR_proJK1_PAD

   Plasmid

   #60630

   Purpose

   Constitutively expresses PAD (phenolic acid decarboxylase)

   Depositor

   Timothy Whitehead

   Article

   Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

   Insert

   Phenolic acid decarboxylase padC (padC )
   

   Expression

   Bacterial

   Mutation

   K146Y

   Promoter

   proJK1

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ825

   Plasmid

   #39315

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSP1D1-Strep-II

   Plasmid

   #224209

   Purpose

   Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

   Depositor

   Anne Robinson

   Article

   Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

   Insert

   MSP1D1-Strep-II
   

   Tags

   Strep-II Tag

   Expression

   Bacterial

   Mutation

   Substitution of 7xHis tag found in pMSP1D1 for a …

   Promoter

   T7

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. FepA

   Plasmid

   #26039

   Depositor

   Johann Deisenhofer

   Article

   Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

   Insert

   FepA (fepA E Coli)
   

   Expression

   Bacterial

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pTSA-13

   Plasmid

   #17327

   Depositor

   Takeshi Sano

   Article

   Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

   Insert

   streptavidin
   

   Expression

   Bacterial

   Mutation

   Truncated to include only amino acids 16-133 of m…

   Available Since

   Feb. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC-ZB

    Plasmid

    #26107

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Tags

    His6 - Z-basic - TEV

    Expression

    Bacterial

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-Tev-WWP2

    Plasmid

    #186807

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-WWP2 (WWP2 Human)
    

    Tags

    His6-Tev

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ALIX-His(1-868, P801G)

    Plasmid

    #186808

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    ALIX-His (1-868, P801G) (PDCD6IP Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. OmpT

    Plasmid

    #213137

    Purpose

    OmpT_native membrane expression

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    OmpT
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Atg24 1B

    Plasmid

    #197046

    Purpose

    His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

    Insert

    Atg24
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCoofy48

    Plasmid

    #55186

    Purpose

    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJ839

    Plasmid

    #39317

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (NMA0631 Neisseria meningitidis)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pHIT179

    Plasmid

    #204510

    Purpose

    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

    Depositor

    Yuji Kohara

    Article

    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

    Insert

    csr-1a (C. elegans) (csr-1 Nematode)
    

    Tags

    6xHis, MBP

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Danio rerio cryaba

    Plasmid

    #47620

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha Ba-crystallin (cryaba Zebrafish)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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