We narrowed to 11,282 results for: Vars

1. pRSETB-de4GFP

   Plasmid

   #85472

   Purpose

   Inducible expression of dual emitting pH sensitive GFP in bacterial cells

   Depositor

   S. James Remington

   Article

   Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

   Insert

   de4GFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S65T/C48S/H148C/T203C

   Promoter

   T7

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   see manuscript

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_PA4114_Cterm_His

   Plasmid

   #220484

   Purpose

   heterologous expression of PA4114 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   PA4114
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKSB-609 U6-sgRNA1 ATCC GGAA

   Plasmid

   #173671

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJYDNgp

   Plasmid

   #162453

   Purpose

   Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-2Glinker-eUnaG2 (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   HA nad myc and eUnaG2 fluorescent protein

   Expression

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GALT-APEX2

   Plasmid

   #215554

   Purpose

   Stable, inducible expression in Golgi

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   APEX2-NES-GalT-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZH516

   Plasmid

   #102669

   Purpose

   Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p1.1

   Plasmid

   #162737

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKSB-610 U6-sgRNA2 GGAA AGAG

   Plasmid

   #173672

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZH514

    Plasmid

    #102667

    Purpose

    Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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