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Plasmid#182078PurposeMammalian Expression of Bassoon scFv. Derived from hybridoma L124/59.DepositorInsertrecombinant mouse scFv targeting Bassoon (Rattus norvegicus) (Bsn Mouse)
UseTagsSortase, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
HNF1B_pLX307
Plasmid#98342PurposeLentiviral expression of HNF1BDepositorInsertHNF1B (HNF1B Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1685
Plasmid#143146PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHNF1B (HNF1B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1684
Plasmid#143965PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHNF1B (HNF1B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bassoon scFv [L124/59]
Plasmid#190508PurposeMammalian Expression of Bassoon scFV. Derived from hybridoma L124/59.DepositorInsertBassoon (Rattus norvegicus) recombinant scFV (Bsn Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KAT6A_ADAM2
Plasmid#205835PurposeExpress mEGFP-tagged fusion protein, KAT6A_ADAM2 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HNF1B-FUS-IDR
Plasmid#183240PurposeExpression of HNF1B fused to a intrinsically disordered regions of the FUS proteinDepositorInsertHNF1 homeobox B (HNF1B Human)
UseTagsFusion to an IDR from the FUS protein and FLAG ta…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)
Plasmid#207357PurposeLentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGR1-P2A-mCherry
Plasmid#203856PurposeTet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertEGR1 (EGR1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Anti-Bassoon [L124/107R]
Plasmid#219452PurposeMammalian Expression Plasmid of anti-Bassoon (Rat) IgG2a R-mAb. Derived from hybridoma L124/107.DepositorInsertAnti-Bassoon (Rattus norvegicus) recombinant mouse monoclonal antibody. (Bsn Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)
Plasmid#207356PurposeLentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)
Plasmid#185448PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationSee Depositor Comments BelowPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla
Plasmid#199551PurposePiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoterDepositorUsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterTRE+CMVmin promoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)
Plasmid#185447PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMS1428
Plasmid#29224PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle168 (POGZ Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-N_RFWD3:1-774
Plasmid#210954PurposeMammalian expression of full-length human RFWD3. N-terminal NanoLuc luciferase tag.DepositorInsertRFWD3:M1-E774 (RFWD3 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_RFWD3:1-774
Plasmid#211103PurposeMammalian expression of full-length human RFWD3. N-terminal HiBiT tag.DepositorInsertRFWD3:M1-E774 (RFWD3 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_RFWD3:1-774
Plasmid#211108PurposeMammalian expression of full-length human RFWD3. C-terminal HiBiT tag.DepositorInsertRFWD3:M1-E774 (RFWD3 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS1091
Plasmid#29025PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle167 (POGZ Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1092
Plasmid#29026PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle168 (POGZ Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only