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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,352 results for: Cas9

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Showing: 4981 - 5000 of 14352 results

  1. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid
    #132560
    Purpose
    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.
    Depositor
    Baisong Lu
    Publication
    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    132560-plasmid-map-sequence-id-259542

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  3. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid
    #140199
    Purpose
    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Promoter
    PgpdA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140199-plasmid-map-sequence-id-309723

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  4. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid
    #121833
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    x-dCas9(3.7)/Dnmt3a-3L (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121833-plasmid-map-sequence-id-237387

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  9. pEvolvR-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249069
    Purpose
    Expresses nucleus-localized nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249069-plasmid-map-sequence-id-496321

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  15. SpCas9-NRCH-ABE8.17-m+V106W

    Plasmid
    #198551
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8.17-m+V106W
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198551-plasmid-map-sequence-id-388896

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  16. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid
    #191388
    Purpose
    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio
    Depositor
    Yannick Doyon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hGEM(1-110)_hSLBP(56_108)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191388-plasmid-map-sequence-id-378110

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  18. pMOD_A-CmYLCV-dCas9-24XGP41

    Plasmid
    #202009
    Purpose
    Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP
    Depositor
    Michael Smanski
    Publication
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-24XGP41
    Use
    Synthetic Biology; Moclo level 1 vector
    Tags
    GS linkers
    Promoter
    CmYLCV
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    202009-plasmid-map-sequence-id-401182

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  19. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid
    #183876
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ANLN sgRNA spacer (ANLN Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    183876-plasmid-map-sequence-id-390709

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  20. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

    Plasmid
    #148859
    Purpose
    Mammalian Expression of HsXRN1-sgRNA
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HsXRN1-sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148859-plasmid-map-sequence-id-478935

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Showing: 4981 - 5000 of 14352 results