Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 12,887 results for: sequence

Showing: 5001 - 5020 of 12887 results

  1. mmu-miR-30a-5p Block-iT design (BLK)

    Plasmid
    #107789
    Purpose
    miR-30a RNAi Expression
    Depositor
    Fabio Penna
    Insert
    Mmu-miR-30a-5p mature sequence (Mir30a Mouse)
    Use
    RNAi
    Tags
    EmGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    107789-plasmid-map-sequence-id-222786

    Enlarge

  2. pGFP CrkII

    Plasmid
    #50730
    Purpose
    mammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    CrkII (CRK Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

    Enlarge

  9. pET-24a-CXCR4

    Plasmid
    #133431
    Purpose
    Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133431-plasmid-map-sequence-id-267724

    Enlarge

  10. mCerulean-Golgi-7

    Plasmid
    #55371
    Purpose
    Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pBJ-mPar3

    Plasmid
    #53230
    Purpose
    mammalian expression of mouse Par3
    Depositor
    Shaun Coughlin
    Insert
    Par3 (F2rl2 Mouse)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-15 (native signal peptide)
    Promoter
    Available since
    Aug. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. KG#865

    Plasmid
    #110912
    Purpose
    Coding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacers
    Depositor
    Kenneth Miller
    Insert
    Auxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110912-plasmid-map-sequence-id-212549

    Enlarge

  14. KG#883

    Plasmid
    #110913
    Purpose
    Coding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a protein
    Depositor
    Kenneth Miller
    Insert
    5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110913-plasmid-map-sequence-id-212540

    Enlarge

  15. pEGB3_alpha2 35:PhyB:VP16 (GB2745)

    Plasmid
    #160630
    Purpose
    TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
    Depositor
    Diego Orzaez
    Insert
    PhyBVP16
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160630-plasmid-map-sequence-id-321969

    Enlarge

  16. pKG194

    Plasmid
    #69757
    Purpose
    Mammalian Expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and eGFP
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pAc5.1B-DmPABPC1-V5His6_D

    Plasmid
    #146093
    Purpose
    Insect Expression of DmPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    DmPABPC1 (pAbp Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one non-silent mutation G26S compared to the sequ…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146093-plasmid-map-sequence-id-362948

    Enlarge

  20. pJK276

    Plasmid
    #73366
    Purpose
    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)
    Depositor
    T. J. Kappock
    Insert
    plastid omega-3 fatty acid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Mutation I231F. Also insert is missing residues 1…
    Promoter
    GAL1
    Available since
    May 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    73366-plasmid-map-sequence-id-135095

    Enlarge

Showing: 5001 - 5020 of 12887 results