Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 14,926 results for: pET

Showing: 5041 - 5060 of 14926 results

  2. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Use
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

    Enlarge

  4. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    Nicholas Levinson, Markus Seeliger, John Chodera
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

    Enlarge

  8. pBtPIPLC His

    Plasmid
    #173808
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    173808-plasmid-map-sequence-id-345681

    Enlarge

  14. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Use
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

    Enlarge

  17. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    User-defined
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

    Enlarge

  18. pSaPIPLC His

    Plasmid
    #173810
    Purpose
    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87
    Insert
    Staphylococcus aureus plc
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173810-plasmid-map-sequence-id-341162

    Enlarge

  20. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

    Enlarge

Showing: 5041 - 5060 of 14926 results