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C122-E54: mRuby3

Plasmid

#162899

Purpose

Standard Gateway entry clone encoding mRuby3 fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMM0494

Plasmid

#128981

Purpose

LEU2 preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

LEU2 5' homology-ConLS-sfGFP dropout-ConR1-LEU2 3' homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG131

Plasmid

#105337

Purpose

Gateway entry clone generation

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-cat

Plasmid

#69933

Purpose

Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-cat

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PlLacO1

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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c7-87/199_HLL

Plasmid

#63122

Purpose

Mm-cpn cysteine double mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_87/199_HLL (NEWENTRY )

Use

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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c7-132/202_HLL

Plasmid

#63123

Purpose

Mm-cpn double cysteine mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_132/202_HLL (NEWENTRY )

Use

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

Promoter

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBA559

Plasmid

#186235

Purpose

pTet-LbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRU42

Plasmid

#167664

Purpose

ENTRY vector for gRNA2 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU41

Plasmid

#167663

Purpose

ENTRY vector for gRNA1 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU44

Plasmid

#167666

Purpose

ENTRY vector for gRNA4 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU43

Plasmid

#167665

Purpose

ENTRY vector for gRNA3 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 EF1a-tTA-2

Plasmid

#26803

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Elongation Factor 1 alpha promoter and tTA transgene

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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EEF1A2 in pENTR

Plasmid

#216585

Purpose

gateway cloning donor vector containing EEF1A2

Depositor

Christopher Loewen, Douglas Allan

Insert

EEF1A2 (EEF1A2 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-hPGK

Plasmid

#82579

Purpose

5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

Human PGK promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Tags

Expression

Mutation

NLS fusion

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-krt5

Plasmid

#82585

Purpose

5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

zebrafish krt5 promoter (krt5 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Tags

Expression

Mutation

Promoter

CMV-SP6

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pME-cas9-2A-mCherry

Plasmid

#107592

Purpose

An entry vector with cas9 -2A-mCherry

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

cas9-2A-mCherry

Use

CRISPR

Tags

2A-mCherry

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-gRNA-EF1a-EGFP-CAAX

Plasmid

#167936

Purpose

gRNA entry vector

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

U6-gRNA backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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