We narrowed to 47,081 results for: Spr

1. dCas13d-BFP

   Plasmid

   #215720

   Purpose

   Catalytically inactive dCasRx with 2A-BFP

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   dCasRx
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK3082

   Plasmid

   #78742

   Purpose

   Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   LbCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGal-BE3

   Plasmid

   #172409

   Purpose

   Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

   Depositor

   Leonid Kruglyak

   Article

   Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

   Insert

   BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   GALL

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDT-SuperFi-Cas9

   Plasmid

   #183630

   Purpose

   Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

   Depositor

   David Taylor

   Article

   Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

   Promoter

   T7

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. sg2.02 16x(MS2) MUC4.1

   Plasmid

   #101154

   Purpose

   gRNA with 16 MCP binding site

   Depositor

   Mazhar Adli

   Article

   Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

   Insert

   MUC4 (MUC4 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p46Cpf1-OP2

   Plasmid

   #98592

   Purpose

   Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

   Depositor

   Qiong Wu

   Article

   Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

   Insert

   FnCpf1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   araBAD

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRS-LacI-dCas9

   Plasmid

   #220194

   Purpose

   mini-Tn7 dCas9 expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   LacI/pLac

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcCas12nHS_RV6_PT1_empty

   Plasmid

   #203810

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6_PT1
   

   Use

   CRISPR

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNS35 (multiAsCas12a piggyBac)

    Plasmid

    #217336

    Purpose

    piggyBac expression of multiAsCas12a

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFnCpf1GG

    Plasmid

    #131010

    Purpose

    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of FnCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL0527 (pDonor)

    Plasmid

    #130634

    Purpose

    Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

    Plasmid

    #59791

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized VP64 dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Available Since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRH2502

    Plasmid

    #84379

    Purpose

    Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    dcas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Aspartate at position 10 changed to Alanine, Hist…

    Promoter

    uv15tetO

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57-GFP-2A-Cas9

    Plasmid

    #170805

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

    Depositor

    Morten Frodin

    Article

    Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-MH6-EsCas13d

    Plasmid

    #108303

    Purpose

    Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    EsCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG Cas9-2A-Citrine

    Plasmid

    #92358

    Purpose

    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY026

    Plasmid

    #84741

    Purpose

    Expresses huAsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180267

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    EGFR sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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