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EC64422

Plasmid

#191768

Purpose

CRISPR mutagenesis in Medicago truncatula

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

Insert

SpCas9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-4X(MS2-PP7)

Plasmid

#121941

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-4X(MS2-PP7)

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SiC-V1

Plasmid

#133041

Purpose

SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNA500

Plasmid

#149640

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

Plasmid

#49772

Purpose

Level 2 construct

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

sgRNA_PDS2-Cas9-sgRNA_PDS1

Use

CRISPR

Expression

Plant

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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5aa SunTag VP64 CLV3

Plasmid

#115485

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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5aa SunTag VP64 AP3

Plasmid

#115484

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMYCO1_SpdCas9_pmcDA1

Plasmid

#204615

Purpose

CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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gRNAy

Plasmid

#168294

Purpose

Expresses gRNA targeting the yellow gene in Drosophila

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

U6.3-gRNA[y]-scaffold

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ173

Plasmid

#98410

Purpose

Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pQCascade(Ptr)-entry(BsaI)

Plasmid

#175579

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)

Expression

Bacterial

Mutation

NO

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ146

Plasmid

#98406

Purpose

Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.leu1 (leu1 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAH237

Plasmid

#121146

Purpose

Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Inserts

gRNA cassette
humanized Streptococcus pyogenes Cas9

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

S.pombe rrk1 and nmt41

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ164

Plasmid

#98408

Purpose

Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.his3 (his3 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Cas12j2

Plasmid

#188498

Purpose

Expresses FLAG tagged Cas12j2

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTrex-b-NLS-hSpCas9

Plasmid

#62543

Purpose

Expression of Cas9 in T. cruzi

Depositor

Rick Tarleton

Article

Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

Insert

NLS-hSpCas9

Use

CRISPR; Trypanosoma cruzi expression

Tags

3xFLAG and NLS

Promoter

Ribo-HX1

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pADH140

Plasmid

#90988

Purpose

NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

C. maltosa LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pJAT57

Plasmid

#204303

Purpose

Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ290

Plasmid

#129670

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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