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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,416 results for: sequence

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Showing: 5121 - 5140 of 13416 results

  1. T-IL2-12

    Plasmid
    #166564
    Purpose
    scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166564-plasmid-map-sequence-id-327819

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  2. Q-IL18-6

    Plasmid
    #166554
    Purpose
    scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166554-plasmid-map-sequence-id-329244

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  4. pT7-V5-SBP-C1-HsDIS3_Z

    Plasmid
    #148137
    Purpose
    Mammalian Expression of HsDIS3
    Depositor
    Elisa Izaurralde
    Insert
    HsDIS3 (DIS3 Human)
    Expression
    Mammalian
    Mutation
    L760F compared to the sequence given by isoform 2…
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148137-plasmid-map-sequence-id-453676

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  5. pUPD2 PhyB (GB2721)

    Plasmid
    #160603
    Purpose
    chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.
    Depositor
    Diego Orzaez
    Insert
    PhyB
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160603-plasmid-map-sequence-id-322052

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  6. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177433-plasmid-map-sequence-id-348464

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  7. Z-AIMP1-1

    Plasmid
    #166560
    Purpose
    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166560-plasmid-map-sequence-id-329268

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  8. G-BRD4-4

    Plasmid
    #166518
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 44-168 of 1362, Bromodomain 1.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166518-plasmid-map-sequence-id-329057

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  9. pAc5.1B-lambdaN-HA-DmARC1_N

    Plasmid
    #146978
    Purpose
    Insect Expression of DmARC1
    Depositor
    Elisa Izaurralde
    Insert
    DmARC1 (Arc1 Fly)
    Expression
    Insect
    Mutation
    5 silent mutations and one non silent mutation G3…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146978-plasmid-map-sequence-id-389936

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  10. pLNHA-C1-HsNot1iso1-shRNAres_V

    Plasmid
    #147749
    Purpose
    Mammalian Expression of HsNot1iso1-shRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1-shRNAres (CNOT1 Human)
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147749-plasmid-map-sequence-id-444359

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  11. pLNCX1 Myr AKT2

    Plasmid
    #27294
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B beta (AKT2 Human)
    Use
    Retroviral
    Tags
    HA and myristolation sequence
    Mutation
    PH domain deleted (aa1-107)
    Available Since
    April 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. AAV Ef1a-mRuby3-WPRE

    Plasmid
    #135427
    Purpose
    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.
    Depositor
    Rylan Larsen
    Insert
    mRuby3
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135427-plasmid-map-sequence-id-271155

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  13. pTH671-SUP35-H348Q

    Plasmid
    #29732
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Expression
    Yeast
    Mutation
    H348Q mutant gene of SUP35 including genomic sequ…
    Available Since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    29732-plasmid-map-sequence-id-13818

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  14. pKG210

    Plasmid
    #69761
    Purpose
    Retroviral expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pKG402

    Plasmid
    #69760
    Purpose
    Retroviral expression of CENP-T aa 256-561
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 256-561 (CENPT Human)
    Use
    Retroviral
    Tags
    mCherry-TEV-S
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. O-IL8-15

    Plasmid
    #166544
    Purpose
    scFv of a human scaffold targeting Interleukin-8. Antigen coverage aa 28-99 of 99, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166544-plasmid-map-sequence-id-328277

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  17. pHBS985 TDP43 RRM-GFP G348C

    Plasmid
    #107832
    Purpose
    To test the effect of sequence on TDP43 droplet properties
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Expression
    Mammalian
    Mutation
    G348C mutation in LLPS reporter
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    107832-plasmid-map-sequence-id-207970

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  18. pKG212

    Plasmid
    #69758
    Purpose
    Retroviral expression of CENP-T aa 1-242
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-242 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pHBS996 TDP43 RRM-GFP A382T

    Plasmid
    #107816
    Purpose
    To test the effect of sequence on TDP43 droplet properties
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Expression
    Mammalian
    Mutation
    A382T mutation in LLPS reporter
    Available Since
    April 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    107816-plasmid-map-sequence-id-208181

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  20. pHBS1051 TDP43 RRM-GFP F-Y

    Plasmid
    #107802
    Purpose
    To test the effect of sequence on TDP43 droplet properties
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Expression
    Mammalian
    Mutation
    All phenylalanine to tyrosine in LLPS reporter
    Available Since
    April 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    107802-plasmid-map-sequence-id-208177

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Showing: 5121 - 5140 of 13416 results