Addgene

We narrowed to 23,361 results for: Nov;

Showing: 7711 - 7740 of 23361 results

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pHAGE2-TetO-Chim-Salhel-I-K57E

Plasmid

#231519

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG K57E flanked with N- and C-termini of mSox2

Use

Lentiviral

Tags

Expression

Mutation

Point mutation at position 57 K to E of the HMG

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi-Q61A/E64M

Plasmid

#231521

Purpose

Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2

Use

Lentiviral

Tags

Expression

Mutation

Point mutations at position 61 Q to A and 64 E to…

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC49-puro

Plasmid

#231989

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-Il1a-ZsGreen

Plasmid

#231987

Purpose

Express IL-1alpha-ZsGreen fusion protein

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

IL-1alpha-ZsGreen fusion construct

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_Del4UC1_EGFP

Plasmid

#229691

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
mutant cavin1b (Del4UC1) (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Del4UC1

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_DrCavin1b_EGFP

Plasmid

#229690

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish Cavin1 (Cavin1 rescue)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
cavin1b (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

LTR viral promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry

Plasmid

#229135

Purpose

Retrovirus driving mCherry reporter

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Insert

mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

Plasmid

#228399

Purpose

Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

3xFLAG-NLS/SV40-AcGFP-NES/PKI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pC130: pAAV.CMV-CasRx-VEGFA sgRNA

Plasmid

#203443

Purpose

Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM150: pAAV.CMV-Cas13e-VEGFA sgRNA array

Plasmid

#203448

Purpose

Plasmid expressing active Cas13e with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_SP-DRD2-V2R-NTEV-tcs-GV-2xHA

Plasmid

#227103

Purpose

DRD2 receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

DRD2-NTEV (DRD2 Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IFr

Plasmid

#192209

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IEr

Plasmid

#192210

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-E Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-ICr

Plasmid

#192211

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-C Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IIAr

Plasmid

#192212

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus II-A Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IEl_Con-IEr

Plasmid

#192213

Purpose

Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-E Leader and Consensus I-E Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-ICl_Con-ICr

Plasmid

#192214

Purpose

Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-C Leader and Consensus I-C Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IIAl_Con-IIAr

Plasmid

#192215

Purpose

Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus II-A Leader and Consensus II-A Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX1-IFl_Con-IFr

Plasmid

#192216

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X1)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX2-IFl_Con-IFr

Plasmid

#192217

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hGRN2

Plasmid

#213683

Purpose

Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

human Granulin-2

Use

AAV

Tags

twin-Strep-FLAG

Expression

Mutation

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

Plasmid

#223965

Purpose

Dual fluorescent reporter for histone H2B and for Cdt1

Depositor

Hee Won Yang

Article

Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

Insert

H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac-mProser1-FLAG-IRES-Blast

Plasmid

#226173

Purpose

Expresses full length mouse PROSER1 with 2X C-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

Insert

Proline Serine Rich protein 1 (Proser1 Mouse)

Use

Tags

2X FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2075

Plasmid

#158098

Purpose

High Copy number plasmid containing smCBA insert.

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

smCBA

Use

Tags

Expression

Mutation

Promoter

smCBA

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMS2156

Plasmid

#158099

Purpose

High Copy number plasmid containing SYN1 insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

SYN1 (SYN1 Human)

Use

Tags

Expression

Mutation

Promoter

SYN1

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pPBbsr2-NRG1-ScNeo

Plasmid

#209911

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Use

Tags

mNeonGreen and mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-mNeonGreen

Plasmid

#209912

Purpose

To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-mNeonGreen (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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