We narrowed to 14,608 results for: SHI

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pZS1[RbsR-T,TreR-T]

Plasmid

#60776

Purpose

Contains PIq driving expression RbsR-T, and PI driving expression of TreR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
TreR-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with RbsR LB…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-T,LacI-T]

Plasmid

#60767

Purpose

Contains PIq driving expression RbsR-T, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
Dimeric LacI with the TAN DBD

Use

Synthetic Biology

Expression

Bacterial

Mutation

Dimeric LacI with the TAN DBD. and LacI/GalR repr…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pKMW121_WT-Cas9_U6-entry

Plasmid

#244818

Purpose

Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW493_U6-sgRNA-entry

Plasmid

#244823

Purpose

Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW122_SpG-Cas9_U6-entry

Plasmid

#244819

Purpose

Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS484_10xHis_MBP_TEV_dSpRY

Plasmid

#244832

Purpose

Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS23_10xHis_MBP_TEV_SpG-Cas9

Plasmid

#244826

Purpose

Bacterial expression of SpG Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS24_10xHis_MBP_TEV_SpRY-Cas9

Plasmid

#244827

Purpose

Bacterial expression of SpRY Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS

Plasmid

#244835

Purpose

Bacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

Plasmid

#191481

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

Depositor

Hao Wu

Article

Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

Insert

SARS-CoV-2 5' UTR ΔSL1

Use

Lentiviral and Luciferase

Mutation

Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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