We narrowed to 13,466 results for: Coli

1. pACYC-Skp-GroEL/ES

   Plasmid

   #83922

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Gro EL/ES
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYC-Skp-TF

   Plasmid

   #83921

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Trigger Factor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS6

   Plasmid

   #55752

   Purpose

   Produces T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   T7 RNA Polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSCX

   Plasmid

   #63918

   Purpose

   Expression of the sucrose utilization phenotype

   Depositor

   Claudia Vickers

   Article

   Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

   Insert

   cscAKB
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL017

   Plasmid

   #53532

   Purpose

   This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpBA
   

   Use

   Tags

   6His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMABC

   Plasmid

   #45398

   Depositor

   Katsu Murakami

   Article

   Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

   Inserts

   rpoA (rpoA Escherichia coli)
   rpoB (rpoB Escherichia coli)
   rpoC (rpoC Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None and T7

   Available Since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL001

   Plasmid

   #115674

   Purpose

   Fluorescent reporter for E.coli K12 fliC promoter activity

   Depositor

   Gene-Wei Li

   Article

   Parker et al J Bacteriol. 2019 Sep 6;201(19). pii: JB

   Insert

   fliC promoter fused with GFP
   

   Use

   Tags

   Green fluorescent protein (GFP)

   Expression

   Bacterial

   Mutation

   Promoter

   fliC promoter

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. EcYajL pET15b

   Plasmid

   #60716

   Purpose

   Recombinant protein expression of YajL

   Depositor

   Mark Wilson

   Article

   Wilson et al J Mol Biol. 2005 Oct 28;353(3):678-91. E

   Insert

   YajL
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAW212

   Plasmid

   #113234

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GGTAG->CCATC

   Promoter

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW228

    Plasmid

    #113242

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->tAggtag

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW227

    Plasmid

    #113241

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->Atggtag

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW218

    Plasmid

    #113240

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW217

    Plasmid

    #113239

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtaC

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAW215

    Plasmid

    #113237

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggAag

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAW214

    Plasmid

    #113236

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->gCtag

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW213

    Plasmid

    #113235

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->Cgtag

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAW211

    Plasmid

    #113233

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->AACGA

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAW216

    Plasmid

    #113238

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtTg

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCASCADE-gltA1-gltA2

    Plasmid

    #71348

    Purpose

    gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    gltA1-gltA2 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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