Addgene

We narrowed to 731 results for: GB1

Showing: 501 - 520 of 731 results

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pJJBAD87

Plasmid

#15353

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CSN)

Use

Tags

GB1

Expression

Bacterial

Mutation

F to S mutation

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD88

Plasmid

#15354

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CGN)

Use

Tags

GB1

Expression

Bacterial

Mutation

F to G mutation

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pGBMCS-SortA

Plasmid

#21931

Depositor

Fuyuhiko Inagaki

Article

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase (srtA Staphylococcus aureus (strain N315))

Use

Tags

GB1 and His-tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only
pIA1364

Plasmid

#172401

Purpose

Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp9 (ORF1ab Synthetic)

Use

Tags

His-GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGBHPS-SortA

Plasmid

#21930

Depositor

Fuyuhiko Inagaki

Article

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase

Use

Tags

GB1, HRV3C cleagage site, and His

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pHBDuet259

Plasmid

#121911

Purpose

NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

NpuDnaBΔ283ΔC39 intein

Use

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain

Promoter

T7

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD113

Plasmid

#121912

Purpose

Charge-Introduced NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBC39 intein

Use

Tags

GB1-His6

Expression

Bacterial

Mutation

S110K, I111K

Promoter

Para

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD168

Plasmid

#121916

Purpose

Oth-NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBC39 intein

Use

Tags

GB1-His6

Expression

Bacterial

Mutation

I111K, E116K

Promoter

Para

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSABAD14-111

Plasmid

#61824

Purpose

NpuDnaB(delta283)dN11 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

NpuDnaB(delta283)dN11 intein

Use

Tags

GB1-H6

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pM198

Plasmid

#173218

Purpose

Expresses OptGFP(1-9)-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

OptGFP(1-9)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHYDuet93

Plasmid

#42303

Depositor

Hideo Iwai

Article

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaEdC6

Use

Tags

H6-GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - L39V)

Plasmid

#136607

Purpose

Expresses DANCER-3 mutant (reversion L39V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (L39V reversion)

Use

Tags

5x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V54I

Promoter

T7 promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-C

Plasmid

#136389

Purpose

Expresses NERD-C static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-C

Use

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-F30L-V39I

Promoter

T7 promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I54V)

Plasmid

#136608

Purpose

Expresses DANCER-3 mutant (reversion I54V)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I54V reversion)

Use

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L

Promoter

T7 promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F34A)

Plasmid

#136606

Purpose

Expresses DANCER-3 mutant (reversion F34A)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F34A reversion)

Use

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-V39L-V54I

Promoter

T7 promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - I7L)

Plasmid

#136605

Purpose

Expresses DANCER-3 mutant (reversion I7L)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (I7L reversion)

Use

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-A34F-V39L-V54I

Promoter

T7 promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (D3 - F3Y)

Plasmid

#136604

Purpose

Expresses DANCER-3 mutant (reversion F3Y)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

DANCER-3 mutant (F3Y reversion)

Use

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSADuet148

Plasmid

#121910

Purpose

Charge-introduced NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290ΔC39 intein

Use

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet116

Plasmid

#121915

Purpose

NpuDnaBΔ290ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290ΔC39 intein

Use

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMHBAD14

Plasmid

#42304

Depositor

Hideo Iwai

Article

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaE C-intein

Use

Tags

GB1-H6

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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