We narrowed to 1,481 results for: PX330

1. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP926-pX330-Batf3

   Plasmid

   #181772

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Batf3 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330 NIPBL gRNA

   Plasmid

   #165590

   Purpose

   Insertion of NIPBL degron

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   NIPBL (Nipbl Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330-UFL1 sgRNA1

   Plasmid

   #134635

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA1 (UFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-GFP-SFPQ

   Plasmid

   #97084

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA GFP-SFPQ
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_ACTB-i4 sgRNA / hSpCas9

   Plasmid

   #172828

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. H513 px330.sgActin.UTR.2

   Plasmid

   #138176

   Purpose

   CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

   Depositor

   Wen Xue

   Article

   Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

   Insert

   sgActin (Actb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Elf5 gRNA

   Plasmid

   #128836

   Purpose

   gRNA for targeting mouse Elf5 loci using CRISPR-cas technique

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   mElf5 gRNA (Elf5 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330 RAD21 gRNA

    Plasmid

    #165592

    Purpose

    Insertion of RAD21 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    RAD21 (Rad21 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-TUBB4B

    Plasmid

    #207785

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330 NL-FKBP-Cas9

    Plasmid

    #162723

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. px330 p300 gRNA

    Plasmid

    #165591

    Purpose

    Insertion of p300 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    P300 (Smarca2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330-CIB1-dCas9

    Plasmid

    #63665

    Purpose

    Mammalian gRNA expression vector also expressing CIB1-dCas9

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and CMV

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid

    #172825

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. px330 RBP1 gRNA

    Plasmid

    #165593

    Purpose

    Insertion of RBP1 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    RBP1 (Polr2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330 N-FKBP-Cas9

    Plasmid

    #162727

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid

    #172834

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-PITCh-H3C2

    Plasmid

    #207780

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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