We narrowed to 6,383 results for: cat.2

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pET15TV-L-hlPYK WT

Plasmid

#187975

Purpose

Expresses the human liver pyruvate kinase

Depositor

Audrey Lamb

Article

McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

Insert

hlPYK WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_hMSH2

Plasmid

#186155

Purpose

sgRNA targeting human MSH2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

MSH2 (MSH2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepHA-SUMO2(Q87R)

Plasmid

#66868

Purpose

Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

Depositor

Ying Liu

Article

Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

Insert

SUMO2 (SUMO2 Human)

Tags

HA and Strep

Expression

Mammalian

Mutation

Q87R

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-reptin

Plasmid

#53609

Purpose

Retroviral expression of human reptin

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

CDS mutated to confer resistance to shRNA. All mu…

Promoter

LTR

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pRK5-PLBD2-6xHis

Plasmid

#213606

Purpose

Expresses PLBD2-6xHis in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

phospholipase B domain containing 2 (PLBD2 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFUGW-ZipACR-EYFP

Plasmid

#88844

Purpose

Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

ZipACR-NotI-EYFP

Use

Lentiviral

Tags

EYFP

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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1161F

Plasmid

#183138

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0121

Plasmid

#177053

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPN022

Plasmid

#91668

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN021

Plasmid

#91667

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

Plasmid

#32735

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
p5A1-Ti

Plasmid

#92018

Purpose

Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Inserts

TALE targeting lac operator with 3 TEV cleavage sites
Catalytically Inactive TEV Protease

Use

TALEN

Tags

5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

Mutation

S219V, C151A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEY105

Plasmid

#223452

Purpose

mgl-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mgl-3 (mgl-3 Nematode)

Expression

Worm

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-LB-geneticin

Plasmid

#213468

Purpose

Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Left border of the TSI locus 1-split fragment of geneticin cassette

Expression

Bacterial

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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HyWnt3-ΔRep:Luc

Plasmid

#192987

Purpose

Plasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Expression

Mammalian

Mutation

Repressor element deleted

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY86

Plasmid

#191079

Purpose

gcy-25 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-25 (gcy-25 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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