We narrowed to 10,083 results for: pET

Showing: 5181 - 5200 of 10083 results

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pETDuet1-LanCL2-H264A-C-Ter-His

Plasmid

#154187

Purpose

To express LanCL2-H264A in bacterial cells

Depositor

Wilfred van der Donk

Publication

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Tags

Hexahistidine

Expression

Bacterial

Mutation

His264->Ala264

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgβ-anticalin-His-ybbr

Plasmid

#168040

Purpose

Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Publication

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag, Fgβ peptide, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-Strep-His E314Amb

Plasmid

#169021

Purpose

Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Publication

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97 (VCP Human)

Tags

His and SBP

Expression

Bacterial

Mutation

E314TAG

Promoter

T7

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_dead_mt

Plasmid

#167270

Purpose

Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Mutation

C145A

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast_dead_mt

Plasmid

#167272

Purpose

Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R-C145A

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3567 (pET151_Spt6 1247-1451 R1282H)

Plasmid

#91816

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed arginine 1282 to histidine; deleted resid…

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3495 (pET151_Spt6 1247-1451 K1435A)

Plasmid

#91817

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1435 to alanine; deleted residues …

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-Ypet-3XNLS-WPRE

Plasmid

#159950

Purpose

nucleus marker

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

Ypet-3XNLS

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-Ypet-optoJNKi-WPRE

Plasmid

#159967

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Publication

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

Ypet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG(C104A/M247C)

Plasmid

#155133

Purpose

Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

Depositor

Sinisa Urban

Publication

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

C104A/M247C, insert codes for GlpG residues 87-276

Promoter

T7

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-Avi-Coh (E154C)-HIS

Plasmid

#153447

Purpose

E. coli expression of Rc. CohE construct with E154C mutation.

Depositor

Michael Nash

Publication

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.CohE

Tags

6xHis and Avi-tag

Expression

Bacterial

Mutation

E154C mutation on CohE

Promoter

T7 promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF H407A H409A H448A

Plasmid

#139566

Purpose

expressed SznF H407A H409A H448A in E. coli

Depositor

Emily Balskus

Publication

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H407A H409A H448A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-S98 (for p24)

Plasmid

#130717

Purpose

Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-S98

Tags

Hisx10 and StrepTag

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97E

Plasmid

#125825

Purpose

Expresses GST-RPN13 (19-132) K97E in E.coli

Depositor

Chris Hill

Publication

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to glutamate

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K83A

Plasmid

#125829

Purpose

Expresses GST-RPN13 (19-132) K83A in E.coli

Depositor

Chris Hill

Publication

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 83 to alanine

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97A

Plasmid

#125823

Purpose

Expresses GST-RPN13 (19-132) K97A in E.coli

Depositor

Chris Hill

Publication

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to alanine

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97D

Plasmid

#125824

Purpose

Expresses GST-RPN13 (19-132) K97D in E.coli

Depositor

Chris Hill

Publication

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to aspartate

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) R104A

Plasmid

#125826

Purpose

Expresses GST-RPN13 (19-132) R104A in E.coli

Depositor

Chris Hill

Publication

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to alanine

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) R104D

Plasmid

#125827

Purpose

Expresses GST-RPN13 (19-132) R104D in E.coli

Depositor

Chris Hill

Publication

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to aspartate

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) R104E

Plasmid

#125828

Purpose

Expresses GST-RPN13 (19-132) R104E in E.coli

Depositor

Chris Hill

Publication

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to glutamate

Promoter

T7

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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