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Plasmid#179403PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_PhdDepositorInsertdPC_Chromo - TAF3_Phd
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC240
Plasmid#118895Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
UseTagsMBP, cleavable with 3C protease and mCherry, clea…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_OTmut
Plasmid#185385PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cellsDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ269C2
Plasmid#164716PurposeGateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editingDepositorInserthAID-zCas9(D10A)-UGI-T2A-MS2-UGI
UseCRISPR; Gateway compatible haid-cbe_v04 entry clo…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEb
Plasmid#193832PurposeExpress TadCBEb (with SpCas9) in mammalian cellsDepositorInsertTadA-CDb-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2903(XI-2 MarkerFree)
Plasmid#73275PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Zeo-eGFP
Plasmid#162743PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC120
Plasmid#118892Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEVDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
UseTagsMBP, cleavable with 3C protease and mGFP, HIS6, c…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Enhancer-ZC3H12D_PromoterWT
Plasmid#153066PurposeZC3H12D promoter region cloned upstream of luciferase reporter geneDepositorInsertZC3H12D promoter region (ZC3H12D Human)
UseLuciferaseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCfB3039(XII-2 MarkerFree)
Plasmid#73279PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 HDV (GB1444)
Plasmid#160576PurposeHepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes.DepositorInsertHDV
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterU6-26Available sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Supt6h-SH2
Plasmid#46518DepositorInsertSupt6h (SUPT6H Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1317-1441PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit
Plasmid#113443PurposeProteinA-mCitrine control expression vectorDepositorInsertProteinA-mCitrine
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS0035
Plasmid#131125PurposeMammalian Target-AID expression vectorDepositorInsertnCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1 and UGIExpressionMammalianMutationD10A in SpCas9PromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F2bbs kan GCANNNAGGk
Plasmid#18752DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGS1022
Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagExpressionMutationPromoterNoneAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc2
Plasmid#186763PurposeAn improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCL6-H2Kk-RV (H2K-BCL6)
Plasmid#40352DepositorInsertBCL6 (BCL6 Human)
UseRetroviralTagsIRES mouse H2KkExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYPQ269A2
Plasmid#164714PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editingDepositorInsertrAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
UseCRISPR; Gateway compatible rapobec1-cbe_v04 entry…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB3036(XI-1 MarkerFree)
Plasmid#73274PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265
Plasmid#164712PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editingDepositorInsertrAPOBEC1-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible rapobec1-cbe_v01 entry…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nck-SH2
Plasmid#46457DepositorInsertNck (NCK1 Human)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 275-377PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcA-SH2
Plasmid#46486DepositorInsertShcA (SHC1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 476-584PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 EBNA1BP2
Plasmid#67072PurposeStandard for cell-free expression benchmarking.DepositorInsertEBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUT1163-HQ858707
Plasmid#27753DepositorInsertbZIP transcription factor from DV507461
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2E1
Plasmid#67089PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2E1 (GTF2E1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t-gfp
Plasmid#48130PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoterDepositorInsertgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
Intersectin I SH3 B domain (human)
Plasmid#47414DepositorInsertIntersectin SH3 domain B (ITSN1 Human)
UseTagsGSTExpressionBacterialMutationonly SH3 domain BPromotertacAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCpf1-BE0
Plasmid#107678PurposeExpresses dLbCpf1-BE0 in mammalian cellsDepositorInsertdLbCpf1-BE0
UseTagsExpressionMammalianMutationD832A/E925/D1148APromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only